MABS2046

Anti-ApoB Antibody, clone 2G11

clone 2G11, from mouse

• Synonym(e): Apolipoprotein B-100
• UNSPSC-Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Eigenschaften

• Biologische Quelle: mouse
• Qualitätsniveau: 100
• Antikörperform: purified immunoglobulin
• Antikörper-Produkttyp: primary antibodies
• Klon: 2G11, monoclonal
• Speziesreaktivität: hamster, mouse, rat
• Darf nicht reagieren mit: human
• Verpackung: antibody small pack of 25 μg
• Methode(n): ELISA: suitable; western blot: suitable
• Isotyp: IgG1κ
• NCBI-Hinterlegungsnummer: NP_033823.2
• UniProt-Hinterlegungsnummer: E9Q414
• Versandbedingung: ambient
• Posttranslationale Modifikation Target: unmodified
• Angaben zum Gen: mouse ... Apob(238055)

Beschreibung

Allgemeine Beschreibung

Apolipoprotein B-100 (UniProt: E9Q414; also known as Apo B-100) is encoded by the Apob gene (Gene ID: 238055) in murine species. Apolipoprotein B is a major protein constituent of chylomicrons. Apo B particles are made up of lipids and proteins and each Apo B is bordered by a phospholipid monolayer with an inner core composed of variable amounts of triglycerides and cholesterol esters. Apo B is synthesized with a signal peptide (aa 1-27) that is subsequently cleaved off. Two isoforms of Apo B are produced- Apo B-100 is synthesized in the liver and Apo B-48 is produced in the intestine. Apo B-100 contains the region that binds to the LDL receptor. It contains VLDL particles, VLDL remnant particles and LDL particles. On the other hand, the Apo B-48 particles are made of chylomicrons and chylomicron remnant particles. Generally, LDL particles account for approximately 90% of Apo B particles in plasma. Abnormal trapping of an Apo B is a major factor in the development of atherosclerotic lesions. When Apo B particles degrade cholesterol is retained with macrophages that induce inflammatory response. Further entry and entrapment of Apo B can promote cardiovascular pathology over time. (Ref.: Sniderman, A et al. (2010). Nat. Rev. Endocrinol. 6(6); 335-346).

Spezifität

Clone 2G11 detects Apolipoprotein B in Mouse, Rat, and Hamster plasma and tissues. It binds to ApoB-48 and ApoB-100, but not to ApoB-39.

Immunogen

ApoB-containing lipoproteins isolated from Apoe -/- Apob 48/48 mice.

Anwendung

Research Category
Zelluläre Signaltransduktion

Detects Apolipoprotein B using this mouse monoclonal Anti-ApoB, clone 2G11 , Cat. No. MABS2046, is a mouse monoclonal antibody that detects Apolipoprotein B in Mouse, Rat, and Hamster and is tested for use in ELISA and Western Blotting.

Western Blotting Analysis: 4 µg/mL from a representative lot detected ApoB in 10 µg of rat liver and mouse liver tissue lysate.

Western Blotting Analysis: A representative lot detected ApoB in Western Blotting applications (Nguyen, A.T., et. al. (2006). Biochem Biophys Acta. 1761(2):182-5; Cheng, D., et. al. (2016). J Biol Chem. 291(45):23793-23803).

ELISA Analysis: A representative lot detected ApoB in ELISA applications (Nguyen, A.T., et. al. (2006). Biochem Biophys Acta. 1761(2):182-5).

Qualität

Evaluated by Western Blotting in rat serum.

Western Blotting Analysis: 4 µg/mL of this antibody detected ApoB in 10 µg of rat serum.

Zielbeschreibung

~260 kDa observed; 509.43 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Physikalische Form

Protein G purified

Format: Purified

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Lagerung und Haltbarkeit

Stable for 1 year at 2-8°C from date of receipt.

Sonstige Hinweise

Concentration: Please refer to lot specific datasheet.

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklassenschlüssel: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flammpunkt (°F): does not flash
• Flammpunkt (°C): does not flash