MABS1726

Anti-MPK-3 (DUSP6) Antibody, clone 5H6.1

clone 5H6.1, from mouse

• Synonym(e): Dual specificity protein phosphatase 6, Dual specificity protein phosphatase PYST1, MAP kinase phosphatase 3, Mitogen-activated protein kinase phosphatase 3, MKP-3
• UNSPSC-Code: 12352203
• eCl@ss: 32160702

Eigenschaften

• Biologische Quelle: mouse
• Qualitätsniveau: 100
• Antikörperform: purified antibody
• Antikörper-Produkttyp: primary antibodies
• Klon: 5H6.1, monoclonal
• Speziesreaktivität: human
• Methode(n): immunohistochemistry: suitable (paraffin); western blot: suitable
• Isotyp: IgG2bκ
• NCBI-Hinterlegungsnummer: NP_001937
• UniProt-Hinterlegungsnummer: Q16828
• Versandbedingung: ambient
• Posttranslationale Modifikation Target: unmodified
• Angaben zum Gen: human ... DUSP6(1848)

Beschreibung

Allgemeine Beschreibung

Dual specificity protein phosphatase 6 (EC 3.1.3.16; EC 3.1.3.48; UniProt Q16828; also known as Dual specificity protein phosphatase PYST1, MAP kinase phosphatase 3, Mitogen-activated protein kinase phosphatase 3, MKP-3) is encoded by the DUSP6 (also known as HH19, MKP3, PYST1) gene (Gene ID 1848) in human. MPK-3 (DUSP6) belongs to the family of dual-specificity phosphatases (DUSPs) that mediate dephosphorylation of MAPKs on threonine/serine and tyrosine residues. DUSP6 is upregulated in HER2+ carcinomas and is among several DUSPs thought to be involved in breast cancer metastasis. DUSP1, DUSP4, and DUSP6 are induced during epithelial-to-mesenchymal transition (EMT) and are involved in forming and maintaining breast cancer stem cells (CSCs), DUSP1 knockdown reduces CSC formation, while DUSP4 or DUSP6 knockdown enhances CSC formation. PKC CK2alpha is reported to phosphorylate DUSP6 and increase its dephosphatase activity. ERK1/2, on the other hand, can phosphorylate DUSP6 to promote its proteosomal degradation.

Spezifität

Clone 5H6.1 targets an epitope within the rhodanese domain present in both spliced isoforms of human MPK-3 (DUSP6) reported by UniProt (Q16828).

Immunogen

GST-tagged recombinant human MPK-3 (DUSP6) rhodanese domain fragment.

Anwendung

Research Category
Zelluläre Signaltransduktion

Detect DUSP6 using this mouse monoclonal Anti-MPK-3 (DUSP6), clone 5H6.1 Antibody, Cat. No. MABS1726, validated for use in Immunohistochemistry (Paraffin) and Western Blotting.

Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected MPK-3 (DUSP6) in human liver, pancreas, and tonsil tissue sections.

Qualität

Evaluated by Western Blotting in HepG2 cell lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected MPK-3 (DUSP6) in 10 µg of HepG2 cell lysate.

Zielbeschreibung

~42 kDa observed. 42.32 kDa (isoform 1) and 26.47 kDa (isoform 2; DUSP6-ALT) calculated. Uncharacterized bands may be observed in some lysate(s).

Physikalische Form

Protein G purified.

Format: Purified

Purified mouse IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Lagerung und Haltbarkeit

Stable for 1 year at 2-8°C from date of receipt.

Sonstige Hinweise

Concentration: Please refer to lot specific datasheet.

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklassenschlüssel: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable