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MABS1152

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Anti-ROR2 Antibody, clone Nt 2535-2835

clone Nt2535-2835, from mouse

Synonym(e):

Tyrosine-protein kinase transmembrane receptor ROR2, mRor2, Ntrkr2, Neurotrophic tyrosine kinase receptor-related 2

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About This Item

UNSPSC-Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

Biologische Quelle

mouse

Qualitätsniveau

Antikörperform

purified immunoglobulin

Antikörper-Produkttyp

primary antibodies

Klon

Nt2535-2835, monoclonal

Speziesreaktivität

mouse, human

Methode(n)

ELISA: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable

Isotyp

IgG1κ

UniProt-Hinterlegungsnummer

Versandbedingung

wet ice

Posttranslationale Modifikation Target

unmodified

Angaben zum Gen

human ... ROR2(4920)

Allgemeine Beschreibung

Tyrosine-protein kinase transmembrane receptor ROR2 (EC 2.7.10.1; UniProt Q9Z138; also known as mRor2, Ntrkr2, Neurotrophic tyrosine kinase receptor-related 2) is encoded by the Ror2 gene (Gene ID 26564) in murine species. Ror2 is a receptor tyrosine kinase that binds Wnt5a and negatively regulates canonical Wnt signaling. Although in C. elegans and Xenopus Ror2 is reported to modulate Wnt signaling nonautonomously by preventing Wnt from stimulating adjacent cells, studies conducted in mammalian cells suggest that Ror2 cytoplasmic domain is crucial for its function and Ror2 inhibits Wnt signaling in a cell-autonomous manner. Ror2 and the closely related Ror1 play essential roles during development. Human ROR2 gene mutations have been associated with dominant Brachydactyly B and recessive Robinow syndrome. Ror2-null mice exhibit skeletal abnormalities similar to the human syndromes. In addition, these mice display heart and lung defects and die perinatally. Ror2 is initially translated with an N-terminal signal sequence (a.a. 1-33), the removal of which yields the mature receptor with an extracellular region (a.a. 34-403), a transmembrane segment (a.a. 404-424), followed by the cytoplasmic region (a.a. 425-944) that contains the kinase domain (a.a. 473-746), a Pro-rich domain (a.a. 784-857) sandwiched between two Ser/Thr-rich domains (a.a. 753-782 and a.a. 859-882).

Spezifität

Clone Nt 2535-2835 is specific to the mouse and human ROR2, but does not detect mouse ROR1. Clone Nt 2535-2835 exclusively detects ROR2 protein in paraffin-embedded tissues (Mikels, A., et al. (2009). J. Biol. Chem. 284(44):30167-30176).

Immunogen

Epitope: Second Ser/Thr-rich domain.
Recombinant protein corresponding to the second Ser/Thr-rich domain of mouse mRor2.

Anwendung

Research Category
Zelluläre Signaltransduktion
Research Sub Category
Kinasen & Phosphatasen
Anti-ROR2 Antibody, clone Nt 2535-2835 is an antibody against ROR2 for use in Western Blotting, Immunohistochemistry, Immunohistochemistry (Paraffin), ELISA.
Immunohistochemistry Analysis: A representative lot detected Ror2 immunoreactivity in parafffin-embedded leiomyosarcoma (LMS) tumor, lung cancer, and breast cancer tissue sections (Courtesy of Dr. Roel Nusse, Stanford University, CA).
Immunohistochemistry Analysis: A representative lot detected Ror2 expression in various regions of wild-type and Ror2+/-, but not Ror2-/-, mouse E13.5 embryos by both fluorescent and non-fluorescent immunohistochemistry using paraformaldehyde-fixed, paraffin-embedded whole mount sections (Mikels, A., et al. (2009). J. Biol. Chem. 284(44):30167-30176).
Immunohistochemistry Analysis: A representative lot detected Ror2 expression in the dermis of wild-type, but not Ror2-knockout mouse skin from E16.5 mouse embryos using paraformaldehyde-fixed, paraffin-embedded whole mount sections (van Amerongen, R., et al. (2012). Dev. Biol. 369(1):101-114).
Western Blotting Analysis: A representative lot detected full-length murine Ror2, as well as Ror2 deletion constructs lacking the first Ser/Thr-rich domain (ST1; a.a 753-782) or the Pro-rich domain (a.a. 784-857), but not Ror2 deletion mutants lacking the ST2 (a.a. 859-882) domain (Mikels, A., et al. (2009). J. Biol. Chem. 284(44):30167-30176).
Enzyme-linked Immunoabsorbent Assay (ELISA): The immunoreactivity of a representative lot toward the immunogen MBP fusion protein was confirmed by ELISA (Mikels, A., et al. (2009). J. Biol. Chem. 284(44):30167-30176).

Qualität

Evaluated by Western Blotting in MEF-1 cell lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected ROR2 in 10 µg of MEF-1 cell lysate.

Zielbeschreibung

~120 kDa observed.

Physikalische Form

Protein G Purified
Format: Purified
Purified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Lagerung und Haltbarkeit

Stable for 1 year at 2-8°C from date of receipt.

Sonstige Hinweise

Concentration: Please refer to lot specific datasheet.

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Lagerklassenschlüssel

12 - Non Combustible Liquids

WGK

WGK 1

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable


Analysenzertifikate (COA)

Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.

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In der Dokumentenbibliothek finden Sie die Dokumentation zu den Produkten, die Sie kürzlich erworben haben.

Die Dokumentenbibliothek aufrufen

Renée van Amerongen et al.
Developmental biology, 369(1), 101-114 (2012-07-10)
Embryonic development is controlled by a small set of signal transduction pathways, with vastly different phenotypic outcomes depending on the time and place of their recruitment. How the same molecular machinery can elicit such specific and distinct responses, remains one
Ror2 receptor requires tyrosine kinase activity to mediate Wnt5A signaling.
Mikels, A; Minami, Y; Nusse, R
The Journal of Biological Chemistry null

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