MABN726

Anti-EphB2 Antibody, clone 2D12C6

ascites fluid, clone 2D12C6, from mouse

• Synonym(e): Ephrin type-B receptor 2, EPHB2 protein
• UNSPSC-Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Eigenschaften

• Biologische Quelle: mouse
• Qualitätsniveau: 100
• Antikörperform: ascites fluid
• Antikörper-Produkttyp: primary antibodies
• Klon: 2D12C6, monoclonal
• Speziesreaktivität: rat, human
• Methode(n): immunocytochemistry: suitable; immunohistochemistry: suitable; western blot: suitable
• Isotyp: IgG2b
• UniProt-Hinterlegungsnummer: Q6NVW1
• Versandbedingung: wet ice
• Posttranslationale Modifikation Target: unmodified
• Angaben zum Gen: human ... EPHB2(2048) , EphB2(2048) ; rat ... EphB2(313633)

Beschreibung

Allgemeine Beschreibung

Ephrin type-B receptor 2 (EphB2) is a receptor tyrosine kinase for ephrin ligands that mediate numerous developmental processes, particularly in the nervous system. Ephrin receptors make up the largest subgroup of the receptor tyrosine kinase (RTK) family. EphB2 is critical for embryonic/postnatal development for lateral suprapyramidal blade formation which is in a specific region of the dentate gyrus. EphB2 is expressed in the two main components of the thymus, developing thymocytes, TEC, colorectal cancer, and in the earliest SGZ progenitor cells as they migrate during development and form the dentate gyrus.

Immunogen

Purified recombinant fragment of EphB2 expressed in E. coli.

Anwendung

Research Category
Neurowissenschaft

Research Sub Category
Entwicklungsneurowissenschaft

Anti-EphB2 Antibody, clone 2D12C6 is a highly specific mouse monoclonal antibody, that targets EphB2 & has been tested in western blotting, IHC & ICC.

Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected EphB2 in HeLa and HepG2 cells.

Immunohistochemistry Analysis: 1:200 to 1:1,000 dilution from a representative lot detected EphB2 in rat cerebellum, pons/midbrain, and hippocampus tissues. Optimal working dilutions must be determined by end user.

Qualität

Evaluated by Western Blot in IMR-32 cell lysate.

Western Blot Analysis: A 1:500 dilution of this antibody detected EphB2 in 10 µg of IMR-32 cell lysate.

Zielbeschreibung

~53 kDa observed

Physikalische Form

Unpurified

Mouse monoclonal IgG2b ascitic fluid containing up to 0.1% sodium azide.

Lagerung und Haltbarkeit

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Hinweis zur Analyse

Control
IMR-32 cell lysate

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklassenschlüssel: 12 - Non Combustible Liquids
• WGK: nwg
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable