MABN381

Anti-APP-C99 Antibody, clone mC99(1-7)

ascites fluid, clone mC99(1-7), from mouse

• Synonym(e): Amyloid beta A4 protein, ABPP, APPI, APP, Alzheimer disease amyloid protein, Cerebral vascular amyloid peptide, CVAP, PreA4, Protease nexin-II, PN-II
• UNSPSC-Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Eigenschaften

• Biologische Quelle: mouse
• Qualitätsniveau: 100
• Antikörperform: ascites fluid
• Antikörper-Produkttyp: primary antibodies
• Klon: mC99(1-7), monoclonal
• Speziesreaktivität: human, mouse
• Methode(n): immunocytochemistry: suitable; immunohistochemistry: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• NCBI-Hinterlegungsnummer: NP_000475
• UniProt-Hinterlegungsnummer: P05067
• Versandbedingung: wet ice
• Posttranslationale Modifikation Target: unmodified
• Angaben zum Gen: human ... APP(351)

Beschreibung

Allgemeine Beschreibung

Amyloid Precursor Protein (APP) is expressed in the brain, kidney, heart, and spleen of fetal tissues; it is induced during neuronal differentiation. The most-substantiated role for APP is in synaptic formation and repair; its expression is upregulated during neuronal differentiation and after neural injury. In adult brain, highest expression of APP is found in the frontal lobe of the cortex. Moderate expression is observed in the cerebellar cortex, the posterior perisylvian cortex-opercular gyri and the temporal associated cortex. Defects in APP are a cause of autosomal dominant Alzheimer′s disease (AD). UP on intracerebroventricular injection of this antibody, A beta levels significantly decreased in the brain of APPPS1 mouse model of AD. A related antibody, Cat. No. MABN380, Anti-APP-C99, clone mC99(70-80) was raised against a slightly different region of the same APP-C99 protein.

Immunogen

Recombinant protein corresponding to Human APP-C99.

Anwendung

Research Category
Neurowissenschaft

Research Sub Category
MMPs & TIMPs

Anti-APP-C99 Antibody, clone mC99(1-7) is a highly specific mouse monoclonal antibody, that targets APP & has been tested in western blotting, IP, ICC & IHC.

Immunoprecipitation Analysis: A representative lot immunoprecipitated APP-C99 in HEK APPSwe cell lysate (Houacine, J., et al. (2012). Neurobiol Aging. 33(11):2704-2714.).

Immunohistochemistry Analysis: A representative lot detected APP-C99 in APPS1 mice and in human Alzheimer brain tissues
(Houacine, J., et al. (2012). Neurobiol Aging. 33(11):2704-2714.).

Immunofluoresence Anlaysis: A representative lot detected APP-C99 in HEK APPSwe cells (Houacine, J., et al. (2012). Neurobiol Aging. 33(11):2704-2714.).

Qualität

Evaluated by Western Blotting in DAPT treated HEK293 cell lysate.

Western Blotting Anlaysis: A 1:2,000 dilution of this antibody detected APP-C99 in 10 µg of DAPT treated HEK293 cell lysate.

Zielbeschreibung

~95 kDa observed. Uncharacterized band(s) may be observed in some cell lysates.

Physikalische Form

Unpurified

Mouse monoclonal IgGκ ascites

Lagerung und Haltbarkeit

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklassenschlüssel: 12 - Non Combustible Liquids
• WGK: nwg
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable