MABN2406

Anti-Tau Antibody, clone TNT-2

clone TNT-2, from mouse

• Synonym(e): Microtubule-associated protein tau, Neurofibrillary tangle protein, Paired helical filament-tau, PHF-tau
• UNSPSC-Code: 12352203
• eCl@ss: 32160702

Eigenschaften

• Biologische Quelle: mouse
• Qualitätsniveau: 100
• Antikörperform: purified immunoglobulin
• Antikörper-Produkttyp: primary antibodies
• Klon: TNT-2, monoclonal
• Speziesreaktivität: rat, human, mouse
• Methode(n): ELISA: suitable; immunofluorescence: suitable; immunohistochemistry: suitable; western blot: suitable
• Isotyp: IgG1κ
• NCBI-Hinterlegungsnummer: NP_058519
• UniProt-Hinterlegungsnummer: P10636
• Versandbedingung: ambient
• Posttranslationale Modifikation Target: unmodified
• Angaben zum Gen: human ... MAPT(4137) ; mouse ... Mapt(17762) ; rat ... Mapt(29477)

Beschreibung

Allgemeine Beschreibung

Microtubule-associated protein tau (UniProt: P10636; also known as Neurofibrillary tangle protein, Paired helical filament-tau, PHF-tau) is encoded by the MAPT (MAPTL, MTBT1, TAU) gene (Gene ID: 4137) in human. Tau proteins are mostly found in the axons of neurons, in the cytosol and in association with plasma membrane components. Normally, Tau promotes microtubule assembly and stability, and might be involved in the establishment and maintenance of neuronal polarity. The C-terminus binds axonal microtubules while the N-terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between both. Axonal polarity is predetermined by TAU/MAPT localization (in the neuronal cell) in the domain of the cell body defined by the centrosome. The localization of Tau protein in the Alzheimer s disease (AD) brain is markedly abnormal and may contribute to neuronal dysfunction. In AD, normal soluble cytoskeletal elements, such as Tau and neurofilaments, are transformed into insoluble paired helical filaments (PHFs). This is linked to the post-translational change in Tau, primarily its hyperphosphorylation by a number of protein kinases. In its non-phosphorylated state, Tau promotes rapid and extensive microtubule (MT) polymerization. A significant consequence of Tau hyperphosphorylation in AD is a reduction in its ability to bind microtubules and promote microtubule assembly. Hyperphosphorylated Tau may contribute to a destabilized microtubule network, impaired axonal transport, and ultimately result in NFT formation and neuronal death.

Spezifität

Clone TNT-2 specirfically detects tau in human, mouse, and rat brain. It targets a sequence of 17 amino acids from the N-terminal region that is common to all isoforms (1-6) of tau.

Immunogen

KLH-conjugated linear peptide corresponding to 17 amino acids from the N-terminal region of human Tau.

Anwendung

Research Category
Neurowissenschaft

Anti-Tau Antibody, clone TNT-2, Cat. No. MABN2406, is a highly specific mouse monoclonal antibody that targets Tau and has been tested in ELISA, Immunofluorescence, Immunohistochemistry, and Western Blotting.

Western Blotting Analysis: 0.02 µg/mL from a representative lot detected Tau in 10 µg of mouse entorhinal cortex.

Immunohistochemistry Analysis: A representative lot detected Tau in Immunohistochemistry applications (Combs, B., et. al. (2016). Neurobiol Dis. 94:18-31).

ELISA Analysis: A representative lot detected Tau in ELISA applications (Combs, B., et. al. (2016). Neurobiol Dis. 94:18-31).

Western Blotting Analysis: A representative lot detected Tau in Western Blotting applications (Combs, B., et. al. (2016). Neurobiol Dis. 94:18-31).

Immunofluorescence Analysis: A representative lot detected Tau in Immunofluorescence applications (Combs, B., et. al. (2016). Neurobiol Dis. 94:18-31).

Qualität

Evaluated by Western Blotting in rat entorhinal cortex.

Western Blotting Analysis: 0.02 µg/mL of this antibody detected Tau in 10 µg of rat entorhinal cortex lysate.

Zielbeschreibung

~55 kda Observed. Uncharacterized bands may be observed in some lysate(s).

Physikalische Form

Protein G purified

Format: Purified

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Lagerung und Haltbarkeit

Stable for 1 year at 2-8°C from date of receipt.

Sonstige Hinweise

Concentration: Please refer to lot specific datasheet.

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklassenschlüssel: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable