MABN1185

Anti-Tau (4-repeat isoform RD4) Antibody, clone 7D12.1

clone 7D12.1, from mouse

• Synonym(e): Microtubule-associated protein tau, 4-repeat isoforms, Neurofibrillary tangle protein, 4-repeat isoforms, Paired helical filament-tau, 4-repeat isoforms, PHF-tau, PNS-tau, Tau-D, Tau-E, Tau-F, Tau-G
• UNSPSC-Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Eigenschaften

• Biologische Quelle: mouse
• Qualitätsniveau: 100
• Antikörperform: purified antibody
• Antikörper-Produkttyp: primary antibodies
• Klon: 7D12.1, monoclonal
• Speziesreaktivität: rat, human
• Speziesreaktivität (Voraussage durch Homologie): mouse (based on 100% sequence homology), bovine (based on 100% sequence homology)
• Methode(n): immunohistochemistry: suitable (paraffin); western blot: suitable
• Isotyp: IgG1κ
• NCBI-Hinterlegungsnummer: NP_058519
• UniProt-Hinterlegungsnummer: P10636
• Posttranslationale Modifikation Target: unmodified
• Angaben zum Gen: human ... MAPT(4137) ; mouse ... Mapt(17762) , Mapt(281296) ; rat ... Mapt(29477)

Beschreibung

Allgemeine Beschreibung

Microtubule-associated protein tau (UniProt P10636; also known as Neurofibrillary tangle protein, Paired helical filament-tau, PHF-tau) is encoded by the MAPT (also known as MAPTL, MTBT1, TAU) gene (Gene ID 4137) in human. Extracellular plaque deposits composed of amyloid-β and intracellular neurofibrillary tangles (NFTs) composed of truncated and hyperphosphorylated tau are two neuropathological hallmarks of Alzheimer′s disease (AD). Tau pathology (tauopathy) can cause toxicity when the brain is devoid of amyloid plaques, and tangle pathology correlates better with clinical dementia than amyloid pathology. Abnormal processing of tau by hyperphosphorylation and proteolytic truncation contribute to the toxicity of tau. Tau also undergoes other types of posttranslational modifications, including glycosylation, ubiquitination, glycation, polyamination, nitration, and lysine methylation, which are believed to be important for its non-pathological functions, including polymerization and stabilization of microtubules. Alternative splicings result in multiple Tau isoforms, including five human Tau spliced isoforms with four C-terminal microtubule-binding repeats/domains (RD4) and four spliced isoforms that contain only three microtubule-binding domains (RD3) due to the absence of exon 10-conding sequence. Isoform-specific antibodies are useful tools for analyzing tau isoforms expression and distribution as well as pathological changes in the human brain.

Spezifität

Clone 7D12.1 targets an epitope sequence present in Tau spliced isoforms with four C-terminal microtubule-binding repeats/domains (RD4), including human isoforms PNS-tau, Tau-D, Tau-E, Tau-F, and Tau-G, but not Tau spliced isoforms that contain only three microtubule-binding domains (RD3) due to the absence of exon 10-conding sequence (human isoforms Fetal-tau, Tau-A, Tau-B, and Tau-C).

Immunogen

Epitope: Junction-flanking region coded by adjacent exons 9 & 10.

KLH-conjugated synthetic peptide (VQIINKKLDLSNVQSKC) corresponding to a junction-flanking sequence coded by adjacent exons 9 & 10 of Tau. The immunizing sequence is identical in human, rat, mouse and bovine.

Anwendung

Research Category
Neurowissenschaft

Research Sub Category
Neurodegenerative Krankheiten

Immunohistochemistry Analysis: A 1:50-250 dilution from a representative lot detected Tau 4-repeat isoforms in Alzheimer′s diseased (AD) human brain, as well as human kidney and non-AD brain tissue sections.

This Anti-Tau Antibody, clone 7D12.1 is validated for use in Western Blotting, Immunohistochemistry (Paraffin) for the detection of Tau (4-repeat isoform RD4).

Qualität

Evaluated by Western Blotting in rat brain tissue cytosol lysate.

Western Blotting Analysis: 0.1 µg/mL of this antibody detected Tau 4-repeat isoforms in 10 µg of rat brain tissue cytosol lysate.

Zielbeschreibung

~45-58 kDa observed. 78,80 kDa (human isoform 1; PNS-tau), 39.88 kDa (human isoform 6; Tau-D), 42.84 kDa (human isoform 7; Tau-E), 45.72 kDa (human isoform 8; Tau-F), 80.81 kDa (human isoform 9; Tau-G) calculated (Met1 removed).

Physikalische Form

Protein G purified.

Format: Purified

Purified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Lagerung und Haltbarkeit

Stable for 1 year at 2-8°C from date of receipt.

Sonstige Hinweise

Concentration: Please refer to lot specific datasheet.

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklassenschlüssel: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable