MABE344

Anti-BICC1/Protein Bicaudal C Homolog 1 Antibody, clone 2D9.1

clone 2D9.1, from mouse

• Synonym(e): Protein bicaudal C homolog 1, Bic-C
• UNSPSC-Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Eigenschaften

• Biologische Quelle: mouse
• Qualitätsniveau: 100
• Antikörperform: purified immunoglobulin
• Antikörper-Produkttyp: primary antibodies
• Klon: 2D9.1, monoclonal
• Speziesreaktivität: human, mouse
• Methode(n): immunoprecipitation (IP): suitable; western blot: suitable
• Isotyp: IgG1κ
• NCBI-Hinterlegungsnummer: NP_001073981
• UniProt-Hinterlegungsnummer: Q9H694
• Versandbedingung: wet ice
• Posttranslationale Modifikation Target: unmodified
• Angaben zum Gen: human ... BICC1(80114)

Beschreibung

Allgemeine Beschreibung

Protein bicaudal C homolog 1 (UniProt Q9H694; also known as Bic-C) is encoded by the BICC1 (also known as CYSRD) gene (Gene ID 80114) in human. Drosophila bicaudal C and its mammalian homologs are RNA-binding proteins involved in symmetric distribution and localized translation of mRNAs of many proteins in a wide range of cells and tissues. RNA binding of Bic-C is mediated by N-terminal KH domains (a.a. 132-199 & 284-348), whereas a C-terminal sterile alpha motif (SAM; a.a. 873-936) self-polymerizes and localizes Bic-C at cytoplasmic foci where silencing of bound mRNAs takes place. Defective polymerization results in decreased Bic-C stability and thereby attenuates inhibition of Dishevelled-2 of Wnt/β-catenin pathway. Loss of BICC1 provokes renal and pancreatic cysts as well as ectopic Wnt/β-catenin signaling during visceral left-right patterning due to defective silencing of Bic-C target mRNAs, including adenylate cyclase 6 (AC6).

Spezifität

Clone 2D9.1 reacts with both spliced isoforms of human and murine BICC1 reported by UniProt (Q9H694 & Q99MQ1).

Immunogen

Epitope: N-terminal half.

Recombinant fragment corresponding to the N-terminal half of human BICC1/Protein Bicaudal C Homolog 1.

Anwendung

Research Category
Epigenetik & nukleäre Funktionen

Research Sub Category
RNA-Stoffwechsel & Bindungsproteine

Anti-BICC1 Antibody, clone 2D9.1 is an antibody against Protein Bicaudal C Homolog 1 for use in Western Blotting, Immunoprecipitation.

Western Blotting Analysis: 1 µg/mL from a representative lot detected Bic-C/BICC1 in human HK and murine mIMCD-3 kidney cell lysates (Courtesy of Dr. Thomas Tuschl laboratory, The Rockefeller University, New York, NY)
Immunoprecipitation Analysis: 9 µg from a representative lot immunoprecipitated exogenously expressed human BICC1 isoforms 1 and 2 from 0.5 mL (~50-100 mg) of HEK293 transfectants lysates (Courtesy of Dr. Thomas Tuschl laboratory, The Rockefeller University, New York, NY).

Qualität

Evaluated by Western Blotting of recombinant human BICC1.

Western Blotting Analysis: 0.2 µg/mL of this antibody detected 10 µg of recombinant human BICC1.

Zielbeschreibung

~130 kDa observed. 104.8/105.0 kDa (Human/mouse isoform 1) and 94.29/93.41 kDa (Human/mouse isoform 2) calculated.

Physikalische Form

Protein G Purified

Format: Purified

Purified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Lagerung und Haltbarkeit

Stable for 1 year at 2-8°C from date of receipt.

Sonstige Hinweise

Concentration: Please refer to lot specific datasheet.

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklassenschlüssel: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable