MABE2044

Anti-SPIB Antibody, clone 235D

• Synonym(e): Transcription factor Spi-B
• UNSPSC-Code: 12352203
• NACRES: NA.43
• Klon: 235D, monoclonal
• application: IHC (p); WB
• Speziesreaktivität: human
• Methode(n): immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable; western blot: suitable

Eigenschaften

• Biologische Quelle: mouse
• Qualitätsniveau: 200
• Konjugat: unconjugated
• Antikörperform: purified antibody
• Antikörper-Produkttyp: primary antibodies
• Klon: 235D, monoclonal
• Mol-Gew.: calculated mol wt 28.82 kDa
• Aufgereinigt durch: using protein G
• Speziesreaktivität: human
• Verpackung: antibody small pack of 100 μg
• Methode(n): immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable; western blot: suitable
• Isotyp: IgG1
• Epitopsequenz: Unknown
• Protein-ID-Hinterlegungsnummer: NP_001230927
• UniProt-Hinterlegungsnummer: Q01892
• Versandbedingung: 2-8°C
• Posttranslationale Modifikation Target: unmodified
• Angaben zum Gen: human ... SPIB(6689)

Beschreibung

Allgemeine Beschreibung

Transcription factor Spi-B (UniProt: Q01892; also known as SPIB) is encoded by the SPIB gene (Gene ID: 6689) in human. SPIB is a member of the ETS family of transcription factors that is expressed in mature B cells, T-cell progenitors, and in plasmacytoid dendritic cells. It is reported to be a key regulator of human plasmacytoid dendritic cell development. It may also be required for B-cell receptor (BCR) signaling that is essential for normal B-cell development and antigenic stimulation. It serves as a sequence specific transcriptional activator that binds to the PU-box, a purine-rich DNA sequence (5′-GAGGAA-3′) that can act as a lymphoid-specific enhancer. SPIB contains a weakly acidic N-terminal transactivation domain (TAD; aa 1-31) that is followed by a second TAD (aa 41-61) that is rich in proline, serine, and threonine. Each of these domains may be required for transcriptional activation of a subset of target genes. Overexpression of SPIB is reported in all cases of blastic plasmacytoid dendritic cell (BPDC) neoplasms, but not in its cutaneous mimics. It can be used as a novel immunohistochemical marker for human BPDC neoplasm. (Ref.: Montes-Moreno, S., et al. (2013). Blood. 121(4); 643-647).

Spezifität

Clone 235D is a mouse monoclonal antibody that detects Transcription factor Spi-B (SPIB).

Immunogen

His-tagged recombinant human transcription factor Spi-B (SPIB).

Anwendung

Quality Control Testing

Evaluated by Immunohistochemistry (Paraffin) in human tonsil tissue sections.

Immunohistochemistry (Paraffin) Analysis: A 1:250 dilution of this antibody detected SPIB in human tonsil tissue sections.

Tested Applications

Western Blotting Analysis: A representative lot detected SPIB in Western Blotting application (Montes-Moreno, S., et al. (2013). Blood. 121(4); 643-7).

Immunohistochemistry (Paraffin) Analysis (IHC(P)): A representative lot detected SPIB in Immunohistochemistry application (Montes-Moreno, S., et al. (2013). Blood. 121(4); 643-7).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Anti-SPIB, clone 235D, Cat. No. MABE2044, is a mouse monoclonal antibody that detects transcription factor Spi-B (SPIB) and is tested for use in Immunohistochemistry (Paraffin) and Western Blotting.

Physikalische Form

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Lagerung und Haltbarkeit

Recommended storage: +2°C to +8°C.

Sonstige Hinweise

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklassenschlüssel: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable