MABC640

Anti-MUM1 Antibody, clone 4G10

ascites fluid, clone 4G10, from mouse

• Synonym(e): PWWP domain-containing protein MUM1, Mutated melanoma-associated antigen 1, MUM-1, Protein expandere
• UNSPSC-Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Eigenschaften

• Biologische Quelle: mouse
• Qualitätsniveau: 100
• Antikörperform: ascites fluid
• Antikörper-Produkttyp: primary antibodies
• Klon: 4G10, monoclonal
• Speziesreaktivität: human
• Methode(n): immunohistochemistry: suitable; western blot: suitable
• Isotyp: IgG1
• UniProt-Hinterlegungsnummer: Q2TAK8
• Versandbedingung: wet ice
• Posttranslationale Modifikation Target: unmodified
• Angaben zum Gen: human ... PWWP3A(84939)

Beschreibung

Allgemeine Beschreibung

Mutated melanoma-associated antigen 1 (MUM1), also known as Protein expandere and PWWP domain-containing protein MUM1. MUM1’s normal role is as a DNA and chromatin associated protein that is involved in the cell’s response to DNA damage. It is driven to DNA breaks by its interaction with another DNA chromatin protein, TP53BP1, which it binds. The binding facilitates chromatin relaxation and entry of DNA repair enzymes. MUM1 is required for efficient DNA repair and cellular survival after DNA damage. Interestingly MUM1 was first identified by cloning the chromosomal breakpoints of a multiple myeloma cell line and was identified as a member of the interferon response transcriptional factor gene family (IFR). MUM1 appears to play a critical role in cell proliferation, differentiation and survival of various cancers, as well as monocytes and dendritic cells. MUM1 is expressed in B cells and plasma cells as well and in some populations of T lymphocytes. EMD-Millipore’s Anti-MUM1 mouse monoclonal antibody has been tested in against recombinantly expressed human MUM1 protein and in paraffin embedded immunohistochemistry on human melanoma, non-Hodgkin’s lymphoma and normal uterus tissue sections.

Immunogen

Purified recombinant fragment of human MUM1 expressed in E. Coli.

Anwendung

Research Category
Apoptose & Krebs

Research Sub Category
Apoptose - Weiterführende Produkte

Detect Mutated melanoma-associated antigen 1 using this mouse monoclonal antibody, Anti-MUM1 Antibody, clone 4G10 validated for use in western blotting & IHC.

Immunohistochemistry Analysis: A 1:200-1,000 dilution from a representative lot detected MUM1 in human uterus, melanoma, and non-Hodgkin`s lymphoma tissues.

Optimal working dilutions must be determined by end user.

Qualität

Evaluated by Western Blotting in recombinant MUM1 protein.

Western Blotting Analysis: A 1:500-2000 dilution of this antibody detected recombinant MUM1.

Zielbeschreibung

~55 kDa observed

Physikalische Form

Unpurified

Mouse monoclonal IgG1 ascitic fluid containing up to 0.1% sodium azide.

Lagerung und Haltbarkeit

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Hinweis zur Analyse

Control
Recombinant MUM1 protein

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklassenschlüssel: 12 - Non Combustible Liquids
• WGK: nwg
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable