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Key Documents

MAB5316

Sigma-Aldrich

Anti-Rhodopsin Antibody, clone RET-P1

clone RET-P1, Chemicon®, from mouse

Synonym(e):

Opsin 2

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About This Item

UNSPSC-Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

Biologische Quelle

mouse

Qualitätsniveau

Antikörperform

purified immunoglobulin

Antikörper-Produkttyp

primary antibodies

Klon

RET-P1, monoclonal

Speziesreaktivität

human, mouse, rat

Hersteller/Markenname

Chemicon®

Methode(n)

immunocytochemistry: suitable
western blot: suitable

Isotyp

IgG1

NCBI-Hinterlegungsnummer

UniProt-Hinterlegungsnummer

Versandbedingung

wet ice

Posttranslationale Modifikation Target

unmodified

Angaben zum Gen

human ... RHO(6010)

Spezifität

Reacts with a protein of 39 kDa identified as rhodopsin (opsin). MAB5316 specifically labels the axons and synaptic pedicles of the rods.

CELLULAR LOCALIZATION:

Cytoplasmic

Immunogen

Membrane preparation from adult rat retina.

Anwendung

Research Category
Neurowissenschaft
Research Sub Category
Sensorik & Peripheres Nervensystem (PNS)
Detect Rhodopsin using this Anti-Rhodopsin Antibody, clone RET-P1 validated for use in IC & WB.
Immunoblotting: 1 μg/mL

Immunocytochemistry

Immunohistochemistry (frozen and formalin/paraffin): 1-2 μg/mL. Staining of formalin fixed tissue sections requires boiling the tissue sections in 10mM citrate buffer, pH 6.0 for 10-20 minutes followed by cooling at room temperature for 20 minutes.

Optimal working dilutions must be determined by end user.

Physikalische Form

Format: Purified
Purified immunoglobulin. Liquid in 10 mM PBS, pH 7.4 with 0.2% BSA and 15 mM sodium azide.

Lagerung und Haltbarkeit

Maintain at 2-8°C in undiluted aliquots for up to 6 months.

Hinweis zur Analyse

Control
POSITIVE CONTROL:

IMR-5 cells, brain or retina.

Sonstige Hinweise

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Rechtliche Hinweise

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Empfehlung

Lagerklassenschlüssel

12 - Non Combustible Liquids

WGK

WGK 2

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable


Analysenzertifikate (COA)

Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.

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In der Dokumentenbibliothek finden Sie die Dokumentation zu den Produkten, die Sie kürzlich erworben haben.

Die Dokumentenbibliothek aufrufen

Vibratome sectioning mouse retina to prepare photoreceptor cultures.
Clerin, E; Yang, Y; Forster, V; Fontaine, V; Sahel, JA; Leveillard, T
Journal of Visualized Experiments null
The F-BAR Protein Rapostlin Regulates Dendritic Spine Formation in Hippocampal Neurons.
Wakita Y, Kakimoto T, Katoh H, Negishi M
The Journal of Biological Chemistry null
AHI1 is required for photoreceptor outer segment development and is a modifier for retinal degeneration in nephronophthisis.
Louie, CM; Caridi, G; Lopes, VS; Brancati, F; Kispert, A; Lancaster, MA; Schlossman et al.
Nature Genetics null
Iman Sahly et al.
The Journal of cell biology, 199(2), 381-399 (2012-10-10)
The mechanisms underlying retinal dystrophy in Usher syndrome type I (USH1) remain unknown because mutant mice lacking any of the USH1 proteins-myosin VIIa, harmonin, cadherin-23, protocadherin-15, sans-do not display retinal degeneration. We found here that, in macaque photoreceptor cells, all
An antibody panel for immunohistochemical analysis of the retina in Davidson's-fixed, paraffin-embedded eyes of rats.
Jennifer S McKay,Samantha J Steele,Gulshanara Ahmed,Elizabeth Johnson,Kerry Ratcliffe
Experimental & Toxicologic Pathology null

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