MAB4092

Anti-P-Selectin Glycoprotein Ligand-1 Antibody, clone KPL-1

clone KPL-1, Chemicon^®, from mouse

• Synonym(e): PSGL-1, CD162
• UNSPSC-Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Eigenschaften

• Biologische Quelle: mouse
• Qualitätsniveau: 100
• Antikörperform: purified immunoglobulin
• Antikörper-Produkttyp: primary antibodies
• Klon: KPL-1, monoclonal
• Speziesreaktivität: human
• Darf nicht reagieren mit: rat (Snapp, KR et al 1998; Baisse, B et al 2007), pig (Snapp, KR et al 1998; Baisse, B et al 2007), bovine (Snapp, KR et al 1998; Baisse, B et al 2007), equine (Snapp, KR et al 1998; Baisse, B et al 2007)
• Hersteller/Markenname: Chemicon^®
• Methode(n): flow cytometry: suitable; immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable; immunoprecipitation (IP): suitable; western blot: suitable
• Isotyp: IgG1κ
• NCBI-Hinterlegungsnummer: NM_003006.3
• UniProt-Hinterlegungsnummer: Q14242
• Versandbedingung: wet ice
• Posttranslationale Modifikation Target: unmodified
• Angaben zum Gen: human ... SELPLG(6404)

Beschreibung

Allgemeine Beschreibung

Interactions between P-selectin and P-selectin glycoprotein ligand-1 (PSGL-1) mediate the earliest “rolling” of leukocytes on the lumenal surface of endothelial cells at sites of inflammation. To date, the primary role of PSGL-1 in mediating the interaction between neutrophils and P-selectin has been well documented. MAB4092 completely blocks recognition of PSGL-1 by either P-selectin or by L-selectin, but does not affect leukocyte recognition of E-selectin. Two color flow cytometry of normal leukocytes has demonstrated that PSGL-1 is expressed on essentially all blood neutrophils, NK cells, blood cells, T cells, and monocytes, but PSGL-1 stains B cells at significantly lower levels than other cell types. Variation in tyrosine sulfation during B cell differentiation may affect the stability of B cells to interact with P- and L-selectin. {Snapp, KR et al 1998}.

Spezifität

P-selectin glycoprotein ligand-1 (PSGL-1). The antibody epitope was mapped to a site within a consensus tyrosine sulfation motif of PSGL-1, previously shown to be essential for interaction with P-selectin (and now shown to be essential for recognition of PSGL-1 by L-selectin). {Snapp KR et al. 1998}. The binding of KPL-1 to human PSGL-1 on HL-60 cells does not require sulfation {Thatte, A et al 2002}.

Immunogen

The antibody epitope was mapped to a site within a consensus tyrosine sulfation motif of PSGL-1, previously shown to be essential for interaction with P-selectin (and now shown to be essential for recognition of PSGL-1 by L-selectin).

Anwendung

Research Category
Entzündung & Immunologie

Research Sub Category
Entzündungs- & Autoimmunmechanismen

Detect P-Selectin Glycoprotein Ligand-1 using this Anti-P-Selectin Glycoprotein Ligand-1 Antibody, clone KPL-1 validated for use in FC, IH, IH(P), IP & WB.

Western blot of HL60 whole cell lysates prepared with 1% triton X-100 in 150mM NaCl, 10mM Tris-HCL pH 7.6, 1 mM Ca++, 1 mM Mg++, with 1mmol aprotinin, PMSF,leupeptin, and pepstatin A {Snapp, KR et al 1998}. 1:1000

FACS {Snapp, KR et al 1998}.

Immunoprecipitation: 0.5% triton X-100 in TBS with proteinase inhibitors; 5 µg/500µL of whole cell lysate (350-500mg/mL total protein).

Functional blocking of P-selectin {Snapp KR et al 1998}.

Immunohistochemistry: Fresh frozen, acetone fixed sections

Immunohistochemistry in paraffin embedded tissues: formalin fixed, pretreatment standard citric acid pH 6.0, microwave treatment is recommended 1:50-1:200

Optimal working dilutions must be determined by the end user.

Physikalische Form

Format: Purified

Purified Immunoglobulin. Liquid in 0.02M PB with 0.25M NaCl, pH7.6, 0.1% Sodium Azide.

Lagerung und Haltbarkeit

Maintain at 2-8°C in undiluted aliquots up to 12 months.

Sonstige Hinweise

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Rechtliche Hinweise

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklassenschlüssel: 10 - Combustible liquids
• WGK: WGK 2
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable