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Key Documents

MAB3776

Sigma-Aldrich

Anti-MDM2 Antibody, clone SMP14

clone SMP14, Chemicon®, from mouse

Synonym(e):

Murine Double Minute 2

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About This Item

UNSPSC-Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

Biologische Quelle

mouse

Qualitätsniveau

100

Antikörperform

purified immunoglobulin

Antikörper-Produkttyp

primary antibodies

Klon

SMP14, monoclonal

Speziesreaktivität

rat, human, mouse

Hersteller/Markenname

Chemicon®

Methode(n)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
immunoprecipitation (IP): suitable

Isotyp

IgG1

NCBI-Hinterlegungsnummer

NM_002392.2
NM_006878.2
NM_006879.2
NM_006881.2
NM_006882.2

UniProt-Hinterlegungsnummer

Q00987

Versandbedingung

wet ice

Posttranslationale Modifikation Target

unmodified

Angaben zum Gen

human ... MDM2(4193)
mouse ... Mdm2(17246)
rat ... Mdm2(314856)

Allgemeine Beschreibung

MDM2 is over-expressed in many tumors. Its principal function is the ubiquitination and degradation of p53 tumour suppressor protein. This monoclonal antibody also recognizes a peptide epitope around Thr-216 of murine MDM2 when Thr-216 is unphosphorylated (Zhang & Prives, 2001). SMP14 also cross reacts with some cytokeratins (6, 14 & 16). This is only a problem when working with certain epithelial cells and not fibroblasts.

Spezifität

Recognizes human MDM2.

Immunogen

Synthetic peptide corresponding to residues 154-167 (CSRPSTSSRRRAISE) of human MDM2 cross-linked to KLH using glutaldehyde.

Anwendung

Research Sub Category
Ubiquitin & Ubiquitin-Stoffwechsel

RNA-Bindeproteine (RBP)
Use Anti-MDM2 Antibody, clone SMP14 (Mouse Monoclonal Antibody) validated in IP, IHC, IHC(P) to detect MDM2 also known as Murine Double Minute 2.
Western blot

Immunohistochemistry (Frozen and Paraffin-embedded sections)

Immunoprecipitation

ELISA

Optimal working dilutions must be determined by end user.

Physikalische Form

Format: Purified
Liquid in 0.02M phosphate buffer, 0.25M NaCl, pH 7.6, with 0.1% sodium azide

Hinweis zur Analyse

Control
POSITIVE CONTROL: Breast carcinoma

Rechtliche Hinweise

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

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Lagerklassenschlüssel

10 - Combustible liquids

WGK

WGK 2

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable

Analysenzertifikate (COA)

Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.

Besitzen Sie dieses Produkt bereits?

In der Dokumentenbibliothek finden Sie die Dokumentation zu den Produkten, die Sie kürzlich erworben haben.

Die Dokumentenbibliothek aufrufen

Cyclin a-CDK phosphorylation regulates MDM2 protein interactions.
Zhang, T and Prives, C
The Journal of Biological Chemistry, 276, 29702-29710 (2001)
Richard O Adeyemi et al.
Journal of virology, 86(15), 8328-8332 (2012-05-25)
The DNA damage response to infection with minute virus of mice (MVM) leads to activated p53; however, p21 levels are reduced via a proteasome-mediated mechanism. This loss was sustained, as virus replicated in infected cells held at the G(2)/M border.
Gengze Wu et al.
Circulation, 130(17), 1452-1465 (2014-08-27)
Long noncoding RNAs (lncRNAs) have recently been implicated in many biological processes and diseases. Atherosclerosis is a major risk factor for cardiovascular disease. However, the functional role of lncRNAs in atherosclerosis is largely unknown. We identified lincRNA-p21 as a key
S M Picksley et al.
Oncogene, 9(9), 2523-2529 (1994-09-01)
The function of p53 is modulated by binding to a number of cellular and viral proteins, such as MDM2 and SV40 large T antigen. An initial immunochemical characterization of the p53-MDM2 complex in a rat fibroblast cell line (Clone 6)

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