MAB3576
Anti-Caldesmon Antibody, smooth muscle, clone N5/22
clone N5/22, Chemicon®, from mouse
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About This Item
UNSPSC-Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Empfohlene Produkte
Biologische Quelle
mouse
Qualitätsniveau
Antikörperform
purified antibody
Antikörper-Produkttyp
primary antibodies
Klon
N5/22, monoclonal
Speziesreaktivität
rabbit, bovine, human, pig
Hersteller/Markenname
Chemicon®
Methode(n)
immunocytochemistry: suitable
immunohistochemistry: suitable (paraffin)
immunoprecipitation (IP): suitable
western blot: suitable
Isotyp
IgG1
NCBI-Hinterlegungsnummer
UniProt-Hinterlegungsnummer
Versandbedingung
wet ice
Posttranslationale Modifikation Target
unmodified
Angaben zum Gen
human ... CALD1(800)
Allgemeine Beschreibung
Caldesmon is a protein component of the thin filaments of smooth muscle myofibrils. It
is also localized in the stress fibers of fibroblasts. Caldesmon was identified as a
Ca2+/Calmodulin-binding protein with molecular weight of 120-150kDa (H-Caldesmon)
and 70-80kDa (L-Caldesmon). H-Caldesmon is the primary isoform in smooth muscle
while L-Caldesmon is most abundant in non-muscle cells. Caldesmon is capable of
binding two calmodulin molecules, one at either end of the protein. Additionally,
Caldesmon is an actin, myosin, and tropomyosin-binding protein. Human L-Caldesmon
is a protein of 538 amino acids with mobility of 80kDa. In vitro, L-Caldesmon inhibits
the actomyosin ATPase in an F-Actin-dependent manner. L-Caldesmon may play an
important function in motile processes such as secretion and organelle movement.
is also localized in the stress fibers of fibroblasts. Caldesmon was identified as a
Ca2+/Calmodulin-binding protein with molecular weight of 120-150kDa (H-Caldesmon)
and 70-80kDa (L-Caldesmon). H-Caldesmon is the primary isoform in smooth muscle
while L-Caldesmon is most abundant in non-muscle cells. Caldesmon is capable of
binding two calmodulin molecules, one at either end of the protein. Additionally,
Caldesmon is an actin, myosin, and tropomyosin-binding protein. Human L-Caldesmon
is a protein of 538 amino acids with mobility of 80kDa. In vitro, L-Caldesmon inhibits
the actomyosin ATPase in an F-Actin-dependent manner. L-Caldesmon may play an
important function in motile processes such as secretion and organelle movement.
Spezifität
Caldesmon, smooth muscle. By Western blot the antibody recognizes a protein of 150-kDa.
Immunogen
Crude smooth muscle extract from normal human adult uterus.
Epitope: smooth muscle
Anwendung
Research Category
Stoffwechsel
Stoffwechsel
Research Sub Category
Muskelphysiologie
Muskelphysiologie
Detect Caldesmon using this Anti-Caldesmon Antibody, smooth muscle, clone N5/22 validated for use in IP, WB, IC, IH(P).
Western blot. Suggested blocking buffer is TBS-Tween with 2% BSA. Suggested dilution buffer is TBS-Tween with 0.05% sodium azide. Preferred gel percentage is 7% and/or 4-20% (or similar) gradient gel.
Immunohistochemistry on frozen and paraffin embedded tissue sections. Suggested fixation for frozen tissue sections is acetone fix for 6 minutes at room temperature. For formalin fixed paraffin embedded tissue sections: microwave in 0.01M citrate buffer (pH 6.0) for 8-10 minutes (note that all microwaves differ and adjustments may need to be made) follow with enzyme digestion (0.01% pronase for 10 mintues). Suggested blocking agent is fetal bovine serum. The antibody has also been used successfully on methyl-Carnoy fixed tissue.
Immunocytochemistry
Immunoprecipitation. Suggested extraction buffer is 20 mM Tris-HCl, pH 7.4, 150 mM NaCl, 1% Triton X-100, 0.1% SDS, 0.5% deoxycholic acid-NaCl and 0.5 mM PMSF. Final reaction volume is 1 mL and suggested capture agent is agarose conjugated anti-mouse IgG.
Optimal working dilutions must be determined by the end user.
Immunohistochemistry on frozen and paraffin embedded tissue sections. Suggested fixation for frozen tissue sections is acetone fix for 6 minutes at room temperature. For formalin fixed paraffin embedded tissue sections: microwave in 0.01M citrate buffer (pH 6.0) for 8-10 minutes (note that all microwaves differ and adjustments may need to be made) follow with enzyme digestion (0.01% pronase for 10 mintues). Suggested blocking agent is fetal bovine serum. The antibody has also been used successfully on methyl-Carnoy fixed tissue.
Immunocytochemistry
Immunoprecipitation. Suggested extraction buffer is 20 mM Tris-HCl, pH 7.4, 150 mM NaCl, 1% Triton X-100, 0.1% SDS, 0.5% deoxycholic acid-NaCl and 0.5 mM PMSF. Final reaction volume is 1 mL and suggested capture agent is agarose conjugated anti-mouse IgG.
Optimal working dilutions must be determined by the end user.
Verlinkung
Replaces: 04-590
Physikalische Form
Format: Purified
Liquid in 0.02M Phosphate buffer with 0.25M NaCl and 0.1% sodium azide.
Lagerung und Haltbarkeit
Maintain at 2-8°C in undiluted aliquots up to 6 months after date of receipt
Hinweis zur Analyse
Control
POSITIVE CONTROL:
Smooth muscle (e.g. aterial tunica media). Negative control: any nonmuscle tissue (e.g. arterial tunica adentitial).
POSITIVE CONTROL:
Smooth muscle (e.g. aterial tunica media). Negative control: any nonmuscle tissue (e.g. arterial tunica adentitial).
Sonstige Hinweise
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Rechtliche Hinweise
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Haftungsausschluss
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Lagerklassenschlüssel
12 - Non Combustible Liquids
WGK
WGK 2
Flammpunkt (°F)
Not applicable
Flammpunkt (°C)
Not applicable
Analysenzertifikate (COA)
Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.
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Jonathan Paul et al.
PloS one, 6(6), e21542-e21542 (2011-07-09)
Human myometrium develops phasic contractions during labor. Phosphorylation of caldesmon (h-CaD) and extracellular signal-regulated kinase 1/2 (ERK 1/2) has been implicated in development of these contractions, however the phospho-regulation of these proteins is yet to be examined during periods of
Alvaro Yogi et al.
Biomedicines, 9(7) (2021-08-07)
Synthetic grafts have been developed for vascular bypass surgery, however, the risks of thrombosis and neointimal hyperplasia still limit their use. Tissue engineering with the use of adipose-derived stem cells (ASCs) has shown promise in addressing these limitations. Here we
M G Frid et al.
Developmental biology, 153(2), 185-193 (1992-10-01)
Expression of the regulatory contractile proteins, heavy caldesmon (h-caldesmon) and calponin was studied in human aortic smooth muscle cells (SMCs) during development and compared with the expression of alpha-SM-actin and smooth muscle-myosin heavy chain (SM-MHCs). For this study, novel monoclonal
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