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MAB3516

Sigma-Aldrich

Anti-Heat Shock Protein 70 Antibody, clone W27

clone W27, Chemicon®, from mouse

Synonym(e):

HSP70

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About This Item

UNSPSC-Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

Biologische Quelle

mouse

Qualitätsniveau

100

Antikörperform

purified antibody

Antikörper-Produkttyp

primary antibodies

Klon

W27, monoclonal

Speziesreaktivität

bovine, human, monkey

Hersteller/Markenname

Chemicon®

Methode(n)

immunohistochemistry: suitable (paraffin)
immunoprecipitation (IP): suitable
western blot: suitable

Isotyp

IgG2a

NCBI-Hinterlegungsnummer

NM_002154.3
NM_198431.1

UniProt-Hinterlegungsnummer

P34932

Versandbedingung

wet ice

Posttranslationale Modifikation Target

unmodified

Angaben zum Gen

human ... HSPA4(3308)

Spezifität

Reacts with both the constitutive (HSP73) and inducible (HSP72) forms of HSP70.

POSITIVE CONTROL:

HeLa cells, BT474 cells or breast carcinoma.

Immunogen

HSP70 from HeLa cells.

Anwendung

Immunoblotting: 0.5-1 μg/mL

Immunohistochemistry (frozen and formalin/paraffin): 2-4 μg/mL for 2 hours at room temperature. Staining of formalin fixed tissue sections requires boiling the tissue sections in 10mM citrate buffer, pH 6.0 for 10-20 minutes followed by cooling at room temperature for 20 minutes.

Immunoprecpitation: 2 μg/mg protein lysate (use Protein G)

Flow cytometry

Optimal working dilutions must be determined by end user.
This Anti-Heat Shock Protein 70 Antibody, clone W27 is validated for use in IP, WB, IH(P) for the detection of Heat Shock Protein 70.

Physikalische Form

Format: Purified

Hinweis zur Analyse

Control
POSITIVE CONTROL: BT474, MAD109, RenCa, SK-NEP-1, U937 cells or breast carcinoma.

Sonstige Hinweise

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Rechtliche Hinweise

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

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Lagerklassenschlüssel

12 - Non Combustible Liquids

WGK

WGK 2

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable

Analysenzertifikate (COA)

Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.

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