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Merck

MAB1982

Sigma-Aldrich

Anti-Integrin α6 Antibody, clone MA6

clone MA6, Chemicon®, from rat

Synonym(e):

CD49f

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About This Item

UNSPSC-Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

Biologische Quelle

rat

Qualitätsniveau

Antikörperform

purified immunoglobulin

Antikörper-Produkttyp

primary antibodies

Klon

MA6, monoclonal

Speziesreaktivität

mouse

Hersteller/Markenname

Chemicon®

Methode(n)

flow cytometry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable

Isotyp

IgG2κ

Eignung

not suitable for Western blot

NCBI-Hinterlegungsnummer

UniProt-Hinterlegungsnummer

Versandbedingung

wet ice

Posttranslationale Modifikation Target

unmodified

Angaben zum Gen

human ... ITGA6(3655)

Spezifität

Reacts with the laminin receptor alpha-6beta-1 and alpha-6beta-4 complexes.

Anwendung

Immunoprecipitation, flow cytometry, immunohistochemistry in frozen tissue sections. Product does not detect alpha 6 in Western blot since the epitope most likely involves both alpha 6 and beta 1 integrin subunit. Product does not block alpha 6 integrin mediated cell adhesion to laminin. Activation of integrin signaling properties by MA6 has not been characterized. Immunoprecipitation of VLA-6 from lysate of 10E6 cell equivalent requires 2 μg of MA6. Recommended concentration for flow cytometry - 10 μg/mL, staining of frozen tissue sections - 20 μg/mL.

Optimal working dilutions must be determined by end user.

Cell lines tested by using MA6 to express (6 integrins include: mouse bone marrow-derived mast cells, melanoma cell line B16F1, squamous carcinoma SCC-VII, fibroblast cell line NIH-3T3, splenic T lymphocytes, thymocytes and various T cell lines.
This Anti-Integrin α6 Antibody, clone MA6 is validated for use in FC, IP, IH for the detection of Integrin α6.

Physikalische Form

Format: Purified
Liquid in 0.02M Phosphate buffer, pH 7.6, with 0.25M NaCl and 0.1% sodium azide.

Lagerung und Haltbarkeit

Store at +2-8°C for up to 12 months.

Sonstige Hinweise

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Rechtliche Hinweise

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

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Lagerklassenschlüssel

10 - Combustible liquids

WGK

WGK 2

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable


Analysenzertifikate (COA)

Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.

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Die Dokumentenbibliothek aufrufen

Derivation of a new human embryonic stem cell line, Endeavour-2, and its characterization.
Kuldip S Sidhu,John P Ryan,Justin G Lees,Bernard E Tuch
In Vitro Cellular & Developmental Biology. Animal null
Lipid defect underlies selective skin barrier impairment of an epidermal-specific deletion of Gata-3.
de Guzman Strong, C; Wertz, PW; Wang, C; Yang, F; Meltzer, PS; Andl, T; Millar, SE; Ho et al.
The Journal of cell biology null
Dongren Yang et al.
The Journal of cell biology, 168(4), 655-666 (2005-02-09)
Schwann cells form basal laminae (BLs) containing laminin-2 (Ln-2; heterotrimer alpha2beta1gamma1) and Ln-8 (alpha4beta1gamma1). Loss of Ln-2 in humans and mice carrying alpha2-chain mutations prevents developing Schwann cells from fully defasciculating axons, resulting in partial amyelination. The principal pathogenic mechanism
Naomi Walsh et al.
Journal of experimental & clinical cancer research : CR, 28, 140-140 (2009-10-15)
Factors mediating the invasion of pancreatic cancer cells through the extracellular matrix (ECM) are not fully understood. In this study, sub-populations of the human pancreatic cancer cell line, MiaPaCa-2 were established which displayed differences in invasion, adhesion, anoikis, anchorage-independent growth
D M Sherry et al.
Graefe's archive for clinical and experimental ophthalmology = Albrecht von Graefes Archiv fur klinische und experimentelle Ophthalmologie, 239(4), 278-287 (2001-07-14)
Integrin receptors mediate cell-extracellular matrix interactions and regulate many events, including cell growth, proliferation, and differentiation. Retinal integrins are incompletely understood, although these receptors are potentially important factors in normal retinal function and pathology. Immunocytochemistry was used to localize alpha

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