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MAB1330

Sigma-Aldrich

Anti-Collagen Type II (COL2A1) Antibody

CHEMICON®, mouse monoclonal, COLL-II

Synonym(e):

Anti-ANFH, Anti-AOM, Anti-COL11A3, Anti-SEDC, Anti-STL1

Anmeldenzur Ansicht organisationsspezifischer und vertraglich vereinbarter Preise


About This Item

UNSPSC-Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

Produktbezeichnung

Anti-Collagen Type II Antibody, clone COLL-II, ascites fluid, clone COLL-II, Chemicon®

Biologische Quelle

mouse

Qualitätsniveau

Antikörperform

ascites fluid

Antikörper-Produkttyp

primary antibodies

Klon

COLL-II, monoclonal

Speziesreaktivität

sheep, human, bovine, rat, pig, mouse, canine

Hersteller/Markenname

Chemicon®

Methode(n)

ELISA: suitable
immunohistochemistry: suitable

Isotyp

IgG1

NCBI-Hinterlegungsnummer

UniProt-Hinterlegungsnummer

Versandbedingung

dry ice

Posttranslationale Modifikation Target

unmodified

Angaben zum Gen

human ... COL2A1(1280)

Spezifität

Reacts with both pepsin-solubilized and CNBr-cleaved human and bovine collagen type II. No cross-reactivity with collagen types I, III, V and IX.

Immunogen

Human (cartilage specific) CNBr-cleaved collagen II.

Anwendung

Research Category
Zellstruktur
Research Sub Category
ECM-Proteine
Immunofluorescence: 1:2-1:5 with fresh, frozen or acetone-fixed material. Mild pepsin digestion is recommended to enhance staining.

Western Blot: 1:1000-1:2000 with chemiluminescent detection

ELISA at 1:100-1:500, pepsin treated samples.





Optimal working dilutions must be determined by the end user.
This Anti-Collagen Type II Antibody, clone COLL-II is validated for use in ELISA, IH for the detection of Collagen Type II.

Physikalische Form

Unpurified
Ascites fluid containing no preservatives.

Lagerung und Haltbarkeit

Maintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Hinweis zur Analyse

Control
Cartilage in lung or fetus

Sonstige Hinweise

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Rechtliche Hinweise

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Lagerklassenschlüssel

11 - Combustible Solids

WGK

WGK 1

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable


Analysenzertifikate (COA)

Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.

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In der Dokumentenbibliothek finden Sie die Dokumentation zu den Produkten, die Sie kürzlich erworben haben.

Die Dokumentenbibliothek aufrufen

ALK1 opposes ALK5/Smad3 signaling and expression of extracellular matrix components in human chondrocytes.
Kenneth W Finnson,Wendy L Parker,Peter ten Dijke,Midory Thorikay,Anie Philip
Journal of Bone and Mineral Research null
Alpha-smooth muscle actin expression and structure integrity in chondrogenesis of human mesenchymal stem cells
Hung, Shih-Chieh, et al
Cell and Tissue Research, 324, 457-466 (2006)
Chondrogenesis of human mesenchymal stem cells encapsulated in alginate beads.
Hsiao-Li Ma, Shih-Chieh Hung, Shan-Yang Lin, Yuh-Lien Chen, Wai-Hee Lo
Journal of Biomedical Materials Research Part A null
Preservation of the chondrocyte's pericellular matrix improves cell-induced cartilage formation.
Lucienne A Vonk,Behrouz Zandieh Doulabi,Chunling Huang,Marco N Helder,Vincent Everts,Ruud A Bank
Journal of Cellular Biochemistry null
Structural studies on cartilage collagen employing limited cleavage and solubilization with pepsin.
Miller, E.J.
Biochemistry, 11, 4903-4909 (1972)

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