MAB1254B

Anti-Chymase Antibody, clone B7, biotin conjugated

clone B7, Chemicon^®, from mouse

• Synonym(e): Mast Cell Protease I
• UNSPSC-Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Eigenschaften

• Biologische Quelle: mouse
• Qualitätsniveau: 100
• Konjugat: biotin conjugate
• Antikörperform: culture supernatant
• Antikörper-Produkttyp: primary antibodies
• Klon: B7, monoclonal
• Speziesreaktivität: human
• Hersteller/Markenname: Chemicon^®
• Methode(n): dot blot: suitable; immunohistochemistry: suitable
• Isotyp: IgG1κ
• Eignung: not suitable for Western blot
• NCBI-Hinterlegungsnummer: NM_001836.2
• UniProt-Hinterlegungsnummer: P23946
• Versandbedingung: wet ice
• Posttranslationale Modifikation Target: unmodified
• Angaben zum Gen: human ... CMA1(1215)

Beschreibung

Allgemeine Beschreibung

Mast cell chymase (EC 3.4.21.39), a member of the granzyme subfamily of the S1 family of peptidases, cleaves C-terminal to Phenylalanine, Tyrosine, Tryptophan, and Leucine residues (in order of preference). Chymase is stored in Mast cell granules within the cell and is the major secreated Mast cell protease. Secreated Mast cell chymase is believed to be involved in extracellular matrix degradation, vasoactive peptide generation, and regulation of gland secreation.

Spezifität

Reacts with human mast cell chymase.

Immunogen

Chymase purified from human skin.

Anwendung

Research Category
Entzündung & Immunologie

Research Sub Category
Entzündungs- & Autoimmunmechanismen

Immunohistochemistry at 1:2,000:1:4,000.

For optimal results, tissues or cytospin preparations should be fixed in Carnoy′s Fluid (60% ethanol, 30% chloroform, 10% glacial acetic acid). Not recommended for use on formaldehyde fixed tissue.

Not recommended for use on Western blots.

Optimal working dilutions must be determined by end user.

This Anti-Chymase Antibody, clone B7, biotin conjugated is validated for use in DB, IH for the detection of Chymase.

Physikalische Form

Culture supernatant, purified by ammonium sulfate precipitation and DEAE-Sephacyl chromatography, labeled with biotin. In 0.01M PBS, 15 mg/mL BSA, 0.1% sodium azide, pH 7.1.

Lagerung und Haltbarkeit

Maintain at 2-8°C in undiluted aliquots for up to 12 months from date of receipt.

Hinweis zur Analyse

Control
POSITIVE CONTROL: lung, heart, skin, placenta.

Sonstige Hinweise

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Rechtliche Hinweise

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklassenschlüssel: 12 - Non Combustible Liquids
• WGK: WGK 2
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable