MAB10234-I

Anti-Hepatitis B Virus Surface Antigen Antibody, clone H21F8-1

• UNSPSC-Code: 12352203
• NACRES: NA.74
• Konjugat: unconjugated
• application: IHC (p)
• Klon: H21F8-1, monoclonal
• Speziesreaktivität: virus
• Methode(n): immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable

Eigenschaften

• Biologische Quelle: mouse
• Qualitätsniveau: 200
• Konjugat: unconjugated
• Antikörperform: purified antibody
• Antikörper-Produkttyp: primary antibodies
• Klon: H21F8-1, monoclonal
• Mol-Gew.: calculated mol wt 43.72 kDa; observed mol wt ~43.72 kDa
• Speziesreaktivität: virus
• Verpackung: antibody small pack of 100 μg
• Methode(n): immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
• Isotyp: IgM
• Epitopsequenz: Unknown
• UniProt-Hinterlegungsnummer: A7XR30
• Versandbedingung: 2-8°C
• Posttranslationale Modifikation Target: unmodified
• Angaben zum Gen: vaccinia virus ... S(944569)

Beschreibung

Allgemeine Beschreibung

Large envelope protein (UniProt: A7XR30; also known as L glycoprotein, L-HBsAg, LHB, Large S protein, Large surface protein, Major surface antigen) is encoded by the S (also known as Large S, P, preS1, preS2) gene (Gene ID: 944569) in Hepatitis B virus (HBV). HBV is a small enveloped virus of the Hepadnaviridae family with a partially double-stranded DNA genome approximately 3.2 kb. Hepatitis B surface antigen is a distinctive serological marker of acute or chronic hepatitis B infection and is the first antigen to appear following infection with hepatitis B virus. HBV contains three viral envelope/surface proteins - large surface antigen, middle surface antigen, and small surface antigen. They are expressed from a single open reading frame. The large envelope protein is synthesized with the pre-S region at the cytosolic side of the endoplasmic reticulum and is not N-glycosylated. It is shown to exist in two topological conformations, one which is termed ′external′ or Le-HBsAg and the other ′internal′ or Li-HBsAg. In its external conformation the protein attaches the virus to cell receptors and thereby initiating infection. This attachment induces virion internalization predominantly through caveolin-mediated endocytosis. The large envelope protein also assures fusion between virion membrane and endosomal membrane. In its internal conformation the protein plays a role in virion morphogenesis and mediates the contact with the nucleocapsid like a matrix protein. (Ref.: Wu, CC., et al. (2018). World J. Gastroenterol. 24(31);: 3488 3499).

Spezifität

Clone H21F8-1 is a mouse monoclonal antibody that detects large envelope protein of Hepatitis B virus.

Immunogen

Hepatitis B virus surface antigen (HBsAg).

Anwendung

Quality Control Testing

Evaluated by Immunohistochemistry (Paraffin) in Hepatitis B virus infected liver (HBsAg-positive) tissue sections.

Immunohistochemistry (Paraffin) Analysis: A 1:50 dilution of this antibody detected Hepatitis B Virus Surface Antigen in Hepatitis B virus infected liver (HBsAg-positive) tissue sections.

Anti-Hepatitis B Virus Surface Antigen, clone H21F8-1, Cat. No. MAB10234-I, is a mouse monoclonal antibody that detects Large envelope protein of Hepatitis B virus and is tested for use in Immunohistochemistry (Paraffin).

Physikalische Form

Purified mouse monoclonal antibody IgM in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Lagerung und Haltbarkeit

Recommend storage at +2°C to +8°C. For long term storage antibodies can be kept at -20°C. Avoid repeated freeze-thaws.

Sonstige Hinweise

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklassenschlüssel: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable