GF446

Thermostable bFGF,Human

HumanKine^®, recombinant, Xeno-free, >95% (SDS-PAGE), suitable for cell culture

• Synonym(e): FGF2, prostatropin, Prostatropin, HBGF-2, BFGF
• UNSPSC-Code: 12352202
• eCl@ss: 32160405
• NACRES: NA.75

Eigenschaften

• Produktbezeichnung: HumanKine^® Thermostable bFGF,; Human Recombinant, Engineered bFGF growth factor with enchanced thermostability compared to the wild-type bFGF protein. Allows a less vigorous feeding schedule for human es/ips and neural stem cell culture.
• Qualitätsniveau: 100
• Assay: >95% (SDS-PAGE)
• Hersteller/Markenname: HumanKine^®
• Verunreinigungen: <0.5 μg/mg protein endotoxin (<5 EU/μg)
• UniProt-Hinterlegungsnummer: P09038

Beschreibung

Allgemeine Beschreibung

Product Source: HumanKine growth factors are expressed in a proprietary human 293 protein expression system and are manufactured without the use of any animal products and are considered Xeno-Free.

The HumanKine Thermostable bFGF is engineered with enchanced thermostability compared to the wild-type bFGF protein without modification of biological function.

Fibroblast Growth Factor-basic (bFGF) is a heparin binding growth factor which stimulates the proliferation of a wide variety of cells including mesenchymal, neuroectodermal and endothelial cells. bFGF also exerts a potent angiogenic activity in vivo. Human bFGF is a 17.2 kDa protein containing 154 amino acid residues. bFGF synergizes with the BMP antagonist noggin to sustain undifferentiated proliferation of human embryonic stem (hES) cells under feeder-free conditions.

Anwendung

Research Category
Stammzellforschung

Research Sub Category
Wachstumsfaktoren & -rezeptoren

Engineered bFGF growth factor with enchanced thermostability compared to the wild-type bFGF protein. Allows a less vigorous feeding schedule for human es/ips and neural stem cell culture.

HumanKine products are Xeno-Free

Qualität

Bioactivity assay: Determined by the dose-dependent stimulation of the proliferation of the 3T3 cell line. EC50 is typically ≤0.5 ng/mL.

Physikalische Form

Lyophilized from a 0.2 μm filtered solution of 1X PBS.

Lagerung und Haltbarkeit

Store at -20°C for up to 4 months from date of receipt. Further dilutions should be made into appropriate buffer or medium to which protein has been added.

Rechtliche Hinweise

HumanKine is a registered trademark of Proteintech Group, Inc. and Humanzyme, Inc

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklassenschlüssel: 13 - Non Combustible Solids
• WGK: WGK 3
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable