AM80

Anti-PAR (Ab-1) Mouse mAb (10H)

liquid, ≥95% (SDS-PAGE), clone 10H

• Synonym(e): Anti-Poly (ADP-Ribose)
• UNSPSC-Code: 12352203
• NACRES: NA.41

Eigenschaften

• Biologische Quelle: mouse
• Qualitätsniveau: 100
• Antikörperform: purified antibody
• Antikörper-Produkttyp: primary antibodies
• Klon: 10H, monoclonal
• Assay: ≥95% (SDS-PAGE)
• Form: liquid
• Enthält: ≤0.1% sodium azide as preservative
• Speziesreaktivität: human, Drosophila, rat, mouse
• Hersteller/Markenname: Calbiochem^®
• Lagerbedingungen: OK to freeze; avoid repeated freeze/thaw cycles
• Isotyp: IgG3
• Versandbedingung: wet ice
• Lagertemp.: −20°C
• Posttranslationale Modifikation Target: unmodified

Beschreibung

Allgemeine Beschreibung

Protein A purified mouse monoclonal antibody. Recognizes poly-ADP-ribose.

Recognizes poly(ADP)-ribose (PAR) in etopside-treated HL-60 cells.

This Anti-PAR (Ab-1) Mouse mAb (10H) is validated for use in Immunoblotting, Immunocytochemistry for the detection of PAR (Ab-1).

Immunogen

Bovine

purified poly(ADP-ribose)

Anwendung

Immunoblotting (2.5 µg/ml)

Immunocytochemistry (5-20 µg/ml)

Verpackung

Please refer to vial label for lot-specific concentration.

Warnhinweis

Toxicity: Standard Handling (A)

Physikalische Form

In 100 mM NaCl, 50 mM HEPES, 1% BSA, pH 7.4.

Rekonstituierung

Following initial thaw, aliquot and freeze (-20°C).

Hinweis zur Analyse

Negative Control
Normal mouse IgG

Positive Control
Etoposide-treated HL-60 cells

Sonstige Hinweise

Kawamitsu, H., et al. 1984. Biochemistry23, 3771.

PAR can be induced in HL-60 cells by treatment with 67 µM etoposide for 3.5 h. Antibody should be titrated for optimal results in an individual system.

Rechtliche Hinweise

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Sicherheitshinweise

• Lagerklassenschlüssel: 11 - Combustible Solids
• WGK: WGK 1
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable