ABN438

Anti-ELFN1 Antibody

from rabbit, purified by affinity chromatography

• Synonym(e): Protein ELFN1, Extracellular leucine-rich repeat and fibronectin type-III domain-containing protein 1, Protein phosphatase 1 regulatory subunit 28
• UNSPSC-Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Eigenschaften

• Biologische Quelle: rabbit
• Qualitätsniveau: 300
• Antikörperform: affinity isolated antibody
• Antikörper-Produkttyp: primary antibodies
• Klon: polyclonal
• Aufgereinigt durch: affinity chromatography
• Speziesreaktivität: human
• Speziesreaktivität (Voraussage durch Homologie): hamster (based on 100% sequence homology), monkey (based on 100% sequence homology), chimpanzee (based on 100% sequence homology), primate (based on 100% sequence homology), baboon (based on 100% sequence homology)
• Methode(n): immunohistochemistry: suitable; western blot: suitable
• NCBI-Hinterlegungsnummer: NP_001122108
• UniProt-Hinterlegungsnummer: P0C7U0
• Versandbedingung: wet ice
• Posttranslationale Modifikation Target: unmodified
• Angaben zum Gen: human ... ELFN1(392617)

Beschreibung

Allgemeine Beschreibung

ELFN1 is a postsynaptic protein that regulates circuit dynamics in the CNS by modulating the temporal dynamics of interneuron recruitment. ELFN1 is specifically expressed in excitatory synapses onto orens-lacunosum (OLM) interneurons and acts as a regulator of presynaptic release in directing of the pyramidal-OLM synapses. ELFN1 localizes to excitatory synapses on somatostatin containing OLM interneurons at the plasma membrane, and ELFN1 protein, expressed post-synaptically, can direct the elaboration of specific electrochemical properties of synapses between particular cell types in the hippocampus. ELFN1’s expression is found in distinct subsets of interneurons of the hippocampus and cortex, and also in discrete subsets of cells in the habenula, septum, globus pallidus, dorsal subiculum, amygdala and several other brain regions in the mouse. The protein is also expressed in diverse cell types, including local GABAergic interneurons as well as long-range projecting GABAergic and glutamatergic neurons.

Immunogen

Epitope: Near N-terminal, extracellular domain

KLH-conjugated linear peptide corresponding to human ELFN1 near the N-terminal, extracellular domain.

Anwendung

Research Category
Neurowissenschaft

Research Sub Category
Entwicklungsabhängige Signalübertragung

Immunohistochemistry Analysis: A 1:50-250 dilution from a representative lot detected ELFN1 in human thalamus and rat cerebral cortex tissue.

This Anti-ELFN1 Antibody is validated for use in Western Blotting and Immunohistochemistry for the detection of ELFN1.

Qualität

Evaluated by Western Blotting in U2OS cell lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected ELFN1 in 10 µg of U2OS cell lysate.

Zielbeschreibung

~100 kDa observed

Physikalische Form

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Lagerung und Haltbarkeit

Stable for 1 year at 2-8°C from date of receipt.

Sonstige Hinweise

Concentration: Please refer to lot specific datasheet.

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklassenschlüssel: 10 - Combustible liquids