ABN242

Anti-Pannexin-1/PANX1 Antibody

from rabbit, purified by affinity chromatography

• Synonym(e): Pannexin-1, Innexin
• UNSPSC-Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Eigenschaften

• Biologische Quelle: rabbit
• Qualitätsniveau: 100
• Antikörperform: affinity isolated antibody
• Antikörper-Produkttyp: primary antibodies
• Klon: polyclonal
• Aufgereinigt durch: affinity chromatography
• Speziesreaktivität: human, mouse, rat
• Speziesreaktivität (Voraussage durch Homologie): gorilla (based on 100% sequence homology), chimpanzee (based on 100% sequence homology)
• Methode(n): immunohistochemistry: suitable; western blot: suitable
• NCBI-Hinterlegungsnummer: NP_056183
• UniProt-Hinterlegungsnummer: Q96RD7
• Versandbedingung: wet ice
• Posttranslationale Modifikation Target: unmodified
• Angaben zum Gen: human ... PANX1(24145)

Beschreibung

Allgemeine Beschreibung

Pannexin-1 (UniProt Q96RD7; also known as Innexin) is encoded by the PANX1 (also known as MRS1, PX1) gene (ORF UNQ2529/PRO6028; Gene ID 24145) in human. Pannexin 1 is a high-conductance, voltage-gated channel protein widely expressed in many organs and tissues, including the sensory systems (eye, inner ear, taste buds, and the olfactory epithelium) and both neuronal and non-neuronal cell types. Three pannexin family genes (PANX1-3) have been identified in human and mouse genomes. Pannexin proteins share structural and functional similarities and play important roles in many physiological and pathological processes, such as ATP release, Ca2+ homeostasis, synaptic neurotransmitters release, apoptosis, and immunological response. Pannexin-1 deficiency causes caspase-3 activation and apoptosis induction of hair cells in cochlear spiral limbus (SLM), resulting in hearing loss among Panx1-/- mice. Panx1 is also expressed in the retina, lens and cornea of the eye, where it plays an important role in mediating the crosstalk between immune and neuronal cells. Pannexin-1 channel opening, frequently together with the activation of purinergic receptors by extracellular ATP, is reported to have a great implication in diverse pathological conditions, including ischemia, trauma, HIV infection, inflammation, migraine, and cancer cell growth.

Spezifität

Expected to react with both spliced isoforms reported by UniProt (Q96RD7-1 & Q96RD7-2).

Immunogen

Epitope: C-terminal

GST-tagged recombinant human Pannexin-1/PANX1 C-terminal fragment.

Anwendung

Research Category
Neurowissenschaft

Research Sub Category
Signalübertragung & Neurowissenschaft

Anti-Pannexin-1/PANX1 Antibody is an antibody against Pannexin-1/PANX1 for use in Western Blotting, Immunohistochemistry.

Immunohistochemistry Analysis: A 1:250 dilution from a representative lot detected Pannexin-1/PANX1 in human testis and mouse kidney tissue sections.

Qualität

Evaluated by Western Blotting in human cortex tissue lysate.

Western Blotting Analysis: 0.1 µg/mL of this antibody detected Pannexin-1/PANX1 in 10 µg of human cortex tissue lysate.

Zielbeschreibung

~55 kDa observed. 55-58 kDa (membrane glycosylated) and 43 kDa (cytosolic non-glycosylated) expected. 48.1 kDa (isoform 1) & 47.6 kDa (isoform 2) calculated.

Verlinkung

Replaces: AB9886

Physikalische Form

Affinity purified

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Lagerung und Haltbarkeit

Stable for 1 year at 2-8°C from date of receipt.

Sonstige Hinweise

Concentration: Please refer to lot specific datasheet.

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklassenschlüssel: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable