ABE413
Anti-NF-E2-related factor 2 Antibody
from rabbit, purified by affinity chromatography
Synonym(e):
Nuclear factor erythroid 2-related factor 2, NF-E2-related factor 2, NFE2-related factor 2, HEBP1, Nuclear factor erythroid derived 2-like 2
Anmeldenzur Ansicht organisationsspezifischer und vertraglich vereinbarter Preise
Alle Fotos(3)
About This Item
UNSPSC-Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
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Biologische Quelle
rabbit
Qualitätsniveau
Antikörperform
affinity isolated antibody
Antikörper-Produkttyp
primary antibodies
Klon
polyclonal
Aufgereinigt durch
affinity chromatography
Speziesreaktivität
human
Speziesreaktivität (Voraussage durch Homologie)
mouse (based on 100% sequence homology), chimpanzee (based on 100% sequence homology), rhesus macaque (based on 100% sequence homology), rat (based on 100% sequence homology)
Verpackung
antibody small pack of 25 μg
Methode(n)
immunocytochemistry: suitable
western blot: suitable
NCBI-Hinterlegungsnummer
UniProt-Hinterlegungsnummer
Versandbedingung
ambient
Lagertemp.
2-8°C
Posttranslationale Modifikation Target
unmodified
Angaben zum Gen
human ... NFE2L2(4780)
Allgemeine Beschreibung
NF-E2-related factor 2 (Nuclear factor erythroid 2-related factor 2) is a transcription activator that binds to antioxidant response elements (ARE) in the promoter regions of target genes. NF-E2-related factor 2 mediates the coordinated up-regulation of genes in response to oxidative stress. NF-E2-related factor 2 may also be involved in the transcriptional activation of genes of the beta-globin cluster. NF-E2-related factor 2 is widely expressed, with highest expression in adult muscle, kidney, lung, liver and fetal muscle.
Spezifität
This antibody recognizes the C-terminus of NF-E2 related factor 2
Immunogen
Epitope: C-terminus
KLH-conjugated linear peptide corresponding to the C-terminus of human NF-E2 related factor 2.
Anwendung
Research Category
Epigenetik & nukleäre Funktionen
Epigenetik & nukleäre Funktionen
Research Sub Category
Transkriptionsfaktoren
Transkriptionsfaktoren
Anti-NF-E2-related factor 2 Antibody is a highly specific rabbit polyclonal antibody, that targets NF-E2-related factor 2 & has been tested in western blotting & ICC.
Western Blotting Analysis: 1 µg/mL from a representative lot detected NF-E2 related factor 2 in 10 µg of Jurkat cell lysate.
Immunocytochemistry Analysis: A 1:200 dilution from a representative lot detected NF-E2 related factor 2 in cells untreated and treated with hydrogen peroxide (Note: Cytoplasmic staining is increased under oxidative stress.).
Immunocytochemistry Analysis: A 1:200 dilution from a representative lot detected NF-E2 related factor 2 in cells untreated and treated with hydrogen peroxide (Note: Cytoplasmic staining is increased under oxidative stress.).
Qualität
Evaluated by Western Blotting in Ramos cell lysate.
Western Blotting Analysis: 1 µg/mL of this antibody detected NF-E2 related factor 2 in 10 µg of Ramos cell lysate.
Western Blotting Analysis: 1 µg/mL of this antibody detected NF-E2 related factor 2 in 10 µg of Ramos cell lysate.
Zielbeschreibung
~75 kDa observed. Two isoforms at 68 kDa (isoform 1) and 66 kDa (isoform 2) are known to exist. This protein has been observed at ~75 kDa due to the abundance of acidic residues found in Nrf2, which causes it to migrate anomalously in SDS-PAGE (Abu-Bakar, A., et al. (2007) Drug Metab Dispos. 35(5):787-794.). A ubiquitinated form of NF-E2-related factor 2 may be observed at ~110 kDa (Li, J., et al. (2005). Toxicol Sci. 83(2):313-328.).
Physikalische Form
Affinity purified
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Lagerung und Haltbarkeit
Stable for 1 year at 2-8°C from date of receipt.
Hinweis zur Analyse
Control
Ramos cell lysate
Ramos cell lysate
Sonstige Hinweise
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Haftungsausschluss
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Produkt-Nr.
Beschreibung
Preisangaben
Lagerklassenschlüssel
12 - Non Combustible Liquids
WGK
WGK 1
Flammpunkt (°F)
Not applicable
Flammpunkt (°C)
Not applicable
Analysenzertifikate (COA)
Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.
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