ABE1331

Anti-Zinc finger protein Eos Antibody

from rabbit, purified by affinity chromatography

• Synonym(e): Zinc finger protein Eos, Ikaros family zinc finger protein 4
• UNSPSC-Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Eigenschaften

• Biologische Quelle: rabbit
• Qualitätsniveau: 100
• Antikörperform: affinity isolated antibody
• Antikörper-Produkttyp: primary antibodies
• Klon: polyclonal
• Aufgereinigt durch: affinity chromatography
• Speziesreaktivität: human
• Speziesreaktivität (Voraussage durch Homologie): mouse (based on 100% sequence homology)
• Methode(n): immunocytochemistry: suitable; western blot: suitable
• NCBI-Hinterlegungsnummer: NP_071910
• UniProt-Hinterlegungsnummer: Q9H2S9
• Versandbedingung: wet ice
• Posttranslationale Modifikation Target: unmodified
• Angaben zum Gen: human ... IKZF4(64375)

Beschreibung

Allgemeine Beschreibung

Zinc finger protein Eos, also known as Zinc finger protein Eos, or Ikaros family zinc finger protein 4, and encoded by the gene name IKZF4/ KIAA1782/ ZNFN1A4, is a member of the Ikaros C2H2-type zinc-finger protein family. Zinc finger protein Eos is a DNA-binding protein that binds to the 5′GGGAATRCC-3′ Ikaros-binding sequence. It is also a transcriptional repressor, which interacts with SPI1 and MITF to repress transcription of the CTSK and ACP5 promoters via recruitment of corepressors SIN3A and CTBP2. Zinc finger protein Eos may be involved in the development of central and peripheral nervous systems. Zinc finger protein Eos interacts with other family members: IKZF1, IKZF2, IKZF3 AND IKZF5; as well as CTBP2, SPI1 and MITF. Zinc finger protein Eos is highly expressed in skeletal muscle, and with low levels of expression in heart, thymus, kidney, liver, and spleen. Zinc finger protein Eos is also expressed in the hematopoietic cell lines MOLT-4, NALM-6 and K-562. High level of expression has been detected in THP-1 and M-07e cell lines, which have characteristics of myeloid and early megakaryocytic cells respectively.

Spezifität

Immunogen sequence is 93% homologous to Bovine.

Immunogen

KLH-conjugated linear peptide corresponding to human Zinc finger protein Eos near the C-terminus.

Anwendung

This Anti-Zinc finger protein Eos antibody is validated for use in WB, IC for the detection of Zinc finger protein Eos.

Western Blotting Analysis: 1.0 µg/mL from a representative lot detected Zinc finger protein Eos in 10 µg of human thymus tissue lysate.
Immunocytochemistry Analysis: 1.0 µg/mL from a representative lot detected Zinc finger protein Eos in HepG2 cells.

Qualität

Evaluated by Western Blotting in HepG2 cell lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected Zinc finger protein Eos in 10 µg of HepG2 cell lysate.

Zielbeschreibung

~60 kDa observed. This antibody is predicted to detect both isoforms of Zinc finger protein Eos (~64 & ~59 kDa) based on immunogen sequence. Uncharacterized band(s) may appear in some lysates.

Sonstige Hinweise

Concentration: Please refer to lot specific datasheet.

Sicherheitshinweise

• Lagerklassenschlüssel: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable