ABD119

Anti-TLE1 Antibody

from rabbit, purified by affinity chromatography

• Synonym(e): Transducin-like enhancer protein 1, E(Sp1) homolog, Enhancer of split groucho-like protein 1, ESG1
• UNSPSC-Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.75

Eigenschaften

• Biologische Quelle: rabbit
• Qualitätsniveau: 100
• Antikörperform: affinity isolated antibody
• Antikörper-Produkttyp: primary antibodies
• Klon: polyclonal
• Aufgereinigt durch: affinity chromatography
• Speziesreaktivität: human
• Speziesreaktivität (Voraussage durch Homologie): mouse (based on 100% sequence homology)
• Methode(n): immunocytochemistry: suitable; western blot: suitable
• NCBI-Hinterlegungsnummer: NP_005068
• UniProt-Hinterlegungsnummer: Q04724
• Versandbedingung: wet ice
• Posttranslationale Modifikation Target: unmodified
• Angaben zum Gen: human ... TLE1(7088)

Beschreibung

Allgemeine Beschreibung

Transducin-like enhancer protein 1 (UniProt Q04724; also known as E(Sp1) homolog, Enhancer of split groucho-like protein 1, ESG1) is encoded by the TLE1 gene (Gene ID 7088) in human. TLE proteins interact with HDACs as well as deacetylated histone tails and play an important role in transcription regulation in several signaling pathways, including Ras, Notch, and Wnt. TLE proteins contain a conserved N-terminal glutamine-rich (Q) domain (a.a. 1-131 of TLE1) that mediates TLEs tetramerization as well as TCF/LEF proteins binding, and is essential for chromatin oligomerization. This is followed by a variable central domain sub-divided into a glycine-prolin-rich (GP) region (a.a. 132-199 of TLE1), the CcN (protein kinase CK2/cell cycle-dependent kinase 2/nuclear localization sequence) domain (a.a. 200-268), a serine-proline-rich (SP) region (a.a. 200-268 and 269-450 of TLE1), and a C-terminal WD40 domain with 6 WD repeats (a.a. 470-767 of TLE1) that interact with multiple transcription factors. The GP region binds directly to HDAC1, the CcN region contains consensus CK2 and Cdc2 phosphorylation sites that are modified in a cell cycle-dependent manner, the SP region is phosphorylated by the Ser/Thr kinases HIPK2 and MAPK, which appear to negatively regulate transcription repression activity, and may also interact with basic helix-loop-helix transcription factors. The C-terminal WD40 domain is essential for chromatin condensation and, together with SP, interacts with bHLH proteins.

Spezifität

This polyclonal antibody targets the CcN (protein kinase CK2/cell cycle-dependent kinase 2/nuclear localization sequence) domain of TLE1 and stained cell nuclei by fluorescent immunocytochemistry.

Immunogen

Epitope: CcN domain.

KLH-conjugated linear peptide corresponding to a sequence within the CcN domain of human TLE1.

Anwendung

Research Category
Stammzellforschung

Research Sub Category
Histon-modifizierende Proteine

Immunocytochemistry Analysis: 1 µg/mL from a representative lot detected TLE1 in HeLa cells and H9 human stem cells.

This Anti-TLE1 Antibody is validated for use in Western Blotting, Immunocytochemistry for the detection of TLE1.

Qualität

Evaluated by Western Blotting in H9 cell lysate.

Western Blotting Analysis: 2 µg/mL of this antiobdy detected TLE1 in 10 µg of H9 human stem cell lysate.

Zielbeschreibung

~90 kDa observed. 83.20 kDa calculated.

Physikalische Form

Affinity purified.

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Lagerung und Haltbarkeit

Stable for 1 year at 2-8°C from date of receipt.

Sonstige Hinweise

Concentration: Please refer to lot specific datasheet.

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklassenschlüssel: 12 - Non Combustible Liquids
• WGK: WGK 1