AB9094-I

Anti-Sodium Pump Subunit alpha-2 Antibody

from rabbit, purified by affinity chromatography

• Synonym(e): Sodium/potassium-transporting ATPase subunit alpha-2, Na(+)/K(+) ATPase alpha-2 subunit, Na(+)/K(+) ATPase alpha(+) subunit, Sodium pump subunit alpha-2, Sodium Pump Subunit alpha-2, Anti-Sodium Pump (Na+ pump (Na+-K+-ATPase)) α 2 Isoform, Sodium/po
• UNSPSC-Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Eigenschaften

• Biologische Quelle: rabbit
• Qualitätsniveau: 100
• Antikörperform: affinity isolated antibody
• Antikörper-Produkttyp: primary antibodies
• Klon: polyclonal
• Aufgereinigt durch: affinity chromatography
• Speziesreaktivität: mouse, rat, human
• Speziesreaktivität (Voraussage durch Homologie): porcine (based on 100% sequence homology), bovine (based on 100% sequence homology)
• Methode(n): immunohistochemistry: suitable (paraffin); immunoprecipitation (IP): suitable; western blot: suitable
• NCBI-Hinterlegungsnummer: NP_036637
• UniProt-Hinterlegungsnummer: P06686
• Versandbedingung: wet ice
• Posttranslationale Modifikation Target: unmodified
• Angaben zum Gen: human ... ATP1A2(477) ; rat ... Atp1A2(24212)

Beschreibung

Allgemeine Beschreibung

Sodium/potassium-transporting ATPase subunit alpha-2 (EC 3.6.3.9; UniProt P06686; also known as Na+/K+ -ATPase alpha 2 subunit, Na(+)/K(+) ATPase alpha(+) subunit, Sodium pump subunit alpha-2) is encoded by the Atp1a2 (also known as RATATPA2) gene (Gene ID 24212) in rat. Mammalian Na+/K+ ATPase is a heteromultimeric complex composed of alpha, beta, and gamma subunits. The alpha subunit catalyzes the hydrolysis of ATP coupled with the exchange of Na+ and K+ across the plasma membrane. The alpha subunit contains ten transmembrane domains with both its N- and C-termini in the cytoplasm. More than 50 mutations have been identified affecting the human ATP1A2 and ATP1A3 genes that are known to cause rapid-onset Dystonia Parkinsonism, familial hemiplegic migraine, alternating hemiplegia of childhood, and variants of familial hemiplegic migraine with neurological complications including seizures and various mood disorders.

Spezifität

Reacts with Sodium Pump (Na+ pump (Na+-K+-ATPase)) alpha 2 isoform. The antibody does not cross react with other Na+-K+-ATPase alpha isoforms.

Immunogen

Epitope: Second cytoplasmic domain

KLH-conjugated linear peptide corresponding to the second cytoplasmic domain of rat Sodium Pump Subunit alpha-2.

Anwendung

Research Category
Neurowissenschaft

Research Sub Category
Entwicklungsabhängige Signalübertragung

Detect Sodium Pump Subunit alpha-2 using this Anti-Sodium Pump Subunit alpha-2 Antibody validated for use in Western Blotting, Immunohistochemistry (Paraffin) and Immunoprecipitation.

Immunohistochemistry Analysis (IHC): A representative lot detected Sodium Pump Subunit alpha-2 in human (1:1,000) and rat (1:250) cerebellum tissue sections.

Immunoprecipitation Analysis (IP): 10 ug from a representative lot immunoprecipitated Sodium Pump Subunit alpha-2 in 500 µg of rat brain tissue lysate.

Qualität

Evaluated by Western Blotting in rat brain tissue lysate.

Western Blotting Analysis: A 1:4,000 dilution of this antibody detected Sodium Pump Subunit alpha-2 in 10 µg of rat brain tissue lysate.

Zielbeschreibung

~100 kDa observed

Verlinkung

Replaces: AB9094

Physikalische Form

Affinity purified

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Lagerung und Haltbarkeit

Stable for 1 year at 2-8°C from date of receipt.

Sonstige Hinweise

Concentration: Please refer to lot specific datasheet.

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklassenschlüssel: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable