AB5252

Anti-Glial Derived Neurotrophic Factor Antibody

serum, Chemicon^®

• Synonym(e): GDNF
• UNSPSC-Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Eigenschaften

• Biologische Quelle: sheep
• Qualitätsniveau: 100
• Antikörperform: serum
• Antikörper-Produkttyp: primary antibodies
• Klon: polyclonal
• Speziesreaktivität: human, rat, mouse
• Hersteller/Markenname: Chemicon^®
• Methode(n): western blot: suitable
• NCBI-Hinterlegungsnummer: NM_000514.2; NM_199231.1; NM_199234.1
• UniProt-Hinterlegungsnummer: P39905
• Versandbedingung: dry ice
• Posttranslationale Modifikation Target: unmodified
• Angaben zum Gen: human ... GDNF(2668)

Beschreibung

Spezifität

Specific for GDNF. No cross reactivity with Neurturin.

Immunogen

Recombinant human GDNF.

Anwendung

Research Category
Neurowissenschaft

Research Sub Category
Neurochemie & Neurotrophine

Neuroinflammation & Schmerz

Anti-Glial Derived Neurotrophic Factor Antibody detects level of Glial Derived Neurotrophic Factor & has been published & validated for use in WB.

Immunoblotting: 1:3,000 using TBS (not PBS) and blocking is best performed with IgG free BSA highest quality. Antibody recognizes ~75 kDa band in non-acid-treated serum samples corresponding to the GDNF/GDNFR complex. When samples are acid treated, a prominent 32 kDa (dimer) and weaker 16 kDa monomer are observed. Acid treatment: homogenized tissue samples in PBS are acid-treated by adding 1N HCL until the pH is less than 3.0 (pH paper strips), incubated 15 minutes at room temperature and then neutralized by adding 1N NaOH to pH 7.6.

Optimal working dilutions must be determined by end user.

Physikalische Form

Sheep serum. Lyophilized. Reconstitute with 100 μL of sterile distilled water. Centrifuge to remove any residue. Contains no preservative. Glycerol (1:1) can be added for additional stability.

Lagerung und Haltbarkeit

Maintain lyophilized material at -20°C to -70°C (dry) for up to twelve months. After reconstitution maintain at -20°C in undiluted aliquots for up to six months. Avoid repeated freeze/thaw cycles.

Rechtliche Hinweise

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklassenschlüssel: 11 - Combustible Solids
• WGK: WGK 1
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable