AB5168

Anti-CHAPSYN-110 Antibody

Chemicon^®, from rabbit

• Synonym(e): PSD-93, Discs Large Homolog 2, DLG2
• UNSPSC-Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Eigenschaften

• Biologische Quelle: rabbit
• Antikörperform: purified immunoglobulin
• Antikörper-Produkttyp: primary antibodies
• Klon: polyclonal
• Speziesreaktivität: rat
• Hersteller/Markenname: Chemicon^®
• Methode(n): immunohistochemistry: suitable; western blot: suitable
• NCBI-Hinterlegungsnummer: NM_001364.2
• UniProt-Hinterlegungsnummer: Q15700
• Versandbedingung: wet ice
• Posttranslationale Modifikation Target: unmodified
• Angaben zum Gen: rat ... Dlg2(64053)

Beschreibung

Spezifität

Recognizes a full length chapsyn-110 protein. It has exhibited no cross reactivity with other known related proteins tested so far.

The immunogen sequence is 42 of 44 amino acids identical in human.

Immunogen

GST fusion protein (Smith & Johnson 1988) and of rat chapsyn-110 (amino acids 336-379) (Brenman et al. 1996; Kim et al. 1996).

Anwendung

This Anti-CHAPSYN-110 Antibody is validated for use in WB, IH for the detection of CHAPSYN-110.

Western blot: 1 to 3 μg/mL (1:300-1:1000) using ECL

Dilutions should be made using a carrier protein such as BSA (1-3%)

Optimal working dilutions must be determined by the end user.

Physikalische Form

Format: Purified

Purified immunoglobulin by Protein A. Lyophilized from phosphate buffered saline, pH 7.4, containing 1% BSA, 5% sucrose as a stabilizer and 0.025% sodium azide as a preservative. Reconstitute with 200 μL of sterile deionized water. Centrifuge antibody preparation before use (10,000 xg for 5 min).

Hinweis zur Analyse

Control
CONTROL ANTIGEN: Included free of charge with the antibody is 50 μg of control antigen (lyophilized powder in phosphate buffered saline, pH 7.4, containing 5% sucrose and 0.025% sodium azide). The stock solution of the antigen can be made up using 200 μL of sterile deionized water. For positive control, in Western blot using 10 ng of protein per lane. For negative control, preincubate 1 μg of fusion protein with 1 μg of antibody for one hour at room temperature. Optimal concentrations must be determined by the end user.

Sonstige Hinweise

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Rechtliche Hinweise

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Sicherheitshinweise

• Lagerklassenschlüssel: 11 - Combustible Solids
• WGK: WGK 3