AB3573

Anti-BCL-XL (pS62) Antibody

Chemicon^®, from rabbit

• Synonym(e): Bcl-2-binding component 6, Bcl-XL/Bcl-2-associated death promoter, Bcl-2-like 8 protein, BAD, BBC6, BCL2L8
• UNSPSC-Code: 12352203
• eCl@ss: 32160702

Eigenschaften

• Biologische Quelle: rabbit
• Qualitätsniveau: 100
• Antikörperform: purified antibody
• Antikörper-Produkttyp: primary antibodies
• Klon: polyclonal
• Aufgereinigt durch: affinity chromatography
• Speziesreaktivität: human
• Speziesreaktivität (Voraussage durch Homologie): rat (100% homologous), mouse (100% homologous)
• Hersteller/Markenname: Chemicon^®
• Methode(n): western blot: suitable
• NCBI-Hinterlegungsnummer: NM_004322.2; NM_032989.1
• UniProt-Hinterlegungsnummer: Q92934
• Versandbedingung: dry ice
• Posttranslationale Modifikation Target: phosphorylation (pSer62)
• Angaben zum Gen: human ... BCL2L1(598)

Beschreibung

Allgemeine Beschreibung

Bcl-xL is a ~28 kDa member of Bcl-2 family of proteins and an important regulator of apoptosis. Bcl-xL forms heterodimers with BAX, BAK, and Bcl-2, and its overexpression in tumor cells confers resistance against chemotherapeutic drugs. Bcl-xL is phosphorylated on many sites including serine 62, a critical site for Bcl-xL response to microtubule-damaging drugs such as taxol and vinblastine. Phosphorylation of serine 62 (thought to be mediated by Jun N-terminal stress kinase (JNK) signaling) negatively regulates the anti-apoptotic function of Bcl-xL and controls the growth of neoplastic cells.

Spezifität

Human Bcl-xL. Mouse and rat Bcl-xL (100% conserved) have not been tested, but are expected to react.

Immunogen

The antiserum was produced against a chemically synthesized phosphopeptide derived from the region of human Bcl-xL that contains serine 62. The sequence is conserved in mouse and rat.

Anwendung

Research Category
Apoptose & Krebs

Research Sub Category
BCL2 & Hemmung

Anti-BCL-XL (pS62) Antibody detects level of BCL-XL (pS62) & has been published & validated for use in WB.

For Western blotting applications, we recommend using the antibody at a 1:1000 dilution.

Qualität

Purified from rabbit serum by sequential epitope-specific chromatography. The antibody has been negatively preadsorbed using a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated Bcl-xL. The final product is generated by affinity chromatography using a Bcl-xL-derived peptide that is phosphorylated at serine 62.

Verlinkung

Replaces: 04-432

Physikalische Form

Dulbecco′s phosphate buffered saline (without Mg²⁺ and Ca²⁺), pH 7.3 (+/- 0.1), 50% glycerol, with 1.0 mg/mL BSA (IgG, protease free) as a carrier. 0.05% sodium azide.

Lagerung und Haltbarkeit

Store at -20°C. We recommend a brief centrifugation before opening to settle vial contents. Then, apportion into working aliquots and store at -20°C. For shipment or short-term storage (up to one week), 2-8°C is sufficient.

Hinweis zur Analyse

Control
SK-BR-3 cells treated with vinblastine.

Rechtliche Hinweise

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklassenschlüssel: 10 - Combustible liquids
• WGK: WGK 2