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Key Documents

AB3269P

Sigma-Aldrich

Anti-Adiponectin Antibody

Chemicon®, from rabbit

Synonym(e):

Acrp30, AdipoQ, apM1, GBP28

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About This Item

UNSPSC-Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

Biologische Quelle

rabbit

Qualitätsniveau

Antikörperform

purified immunoglobulin

Antikörper-Produkttyp

primary antibodies

Klon

polyclonal

Speziesreaktivität

mouse

Hersteller/Markenname

Chemicon®

Methode(n)

ELISA: suitable
western blot: suitable

NCBI-Hinterlegungsnummer

UniProt-Hinterlegungsnummer

Versandbedingung

dry ice

Posttranslationale Modifikation Target

unmodified

Angaben zum Gen

human ... ADIPOQ(9370)

Spezifität

Recognizes mouse adiponectin. Recognizes both natural and recombinant protein.

SPECIES REACTIVITIES:

There is weak reactivity with rat. Reactivity with other species has not been confirmed.

Immunogen

Recombinant mouse adiponectin from E. coli.

Anwendung

Research Category
Stoffwechsel
Research Sub Category
Lipidstoffwechsel & Gewichtsregulierung
Anti-Adiponectin Antibody detects level of Adiponectin & has been published & validated for use in ELISA & WB.
Western blot: 1:5,000-1:10,000 using ECL. Reacts with the ~35 kDa adiponectin protein. Suggested blocking and dilution buffer is TBST with 0.05% Tween 20 with 5% skim milk. Suggested incubation time is 1 hour at room temperature.

ELISA, direct and indirect: 1:5,000-1:10,000.

Optimal working dilutions must be determined by the end user.

Verlinkung

Replaces: MABS176

Physikalische Form

Format: Purified
Purified immunoglobulin in PBS, pH 7.4.

Lagerung und Haltbarkeit

Maintain at -20°C in undiluted aliquots for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles.

Sonstige Hinweise

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Rechtliche Hinweise

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Lagerklassenschlüssel

12 - Non Combustible Liquids

WGK

WGK 2

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable


Analysenzertifikate (COA)

Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.

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Die Dokumentenbibliothek aufrufen

Adiponectin opposes endothelin-1-mediated vasoconstriction in the perfused rat hindlimb.
Bussey, CT; Kolka, CM; Rattigan, S; Richards, SM
American Journal of Physiology. Heart and Circulatory Physiology null
Jiung-Pang Huang et al.
Journal of molecular endocrinology, 60(4), 307-321 (2018-03-28)
Many studies have reported the causes of obese metabolic syndrome (MS); however, the causes of nonobese MS (NMS) remain unknown. In this study, we demonstrated that inflamed dysfunctional adipose tissue plays a crucial role in cholesterol-induced NMS. Control (C), high
Ruben Garcia-Martin et al.
Cell reports, 38(3), 110277-110277 (2022-01-20)
Exosomes/small extracellular vesicles (sEVs) can serve as multifactorial mediators of cell-to-cell communication through their miRNA and protein cargo. Quantitative proteomic analysis of five cell lines representing metabolically important tissues reveals that each cell type has a unique sEV proteome. While

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