AB2226

Anti-MDGA1 Antibody

from rabbit, purified by affinity chromatography

• Synonym(e): MAM domain-containing glycosylphosphatidylinositol anchor protein 1, GPI and MAM protein, GPIM, Glycosylphosphatidylinositol-MAM, MAM domain-containing protein 3
• UNSPSC-Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Eigenschaften

• Biologische Quelle: rabbit
• Qualitätsniveau: 100
• Antikörperform: affinity isolated antibody
• Antikörper-Produkttyp: primary antibodies
• Klon: polyclonal
• Aufgereinigt durch: affinity chromatography
• Speziesreaktivität: mouse, rat
• Speziesreaktivität (Voraussage durch Homologie): human (based on 100% sequence homology), bovine (based on 100% sequence homology)
• Methode(n): western blot: suitable
• NCBI-Hinterlegungsnummer: NP_705691
• UniProt-Hinterlegungsnummer: Q8NFP4
• Versandbedingung: wet ice
• Posttranslationale Modifikation Target: unmodified
• Angaben zum Gen: human ... MDGA1(266727)

Beschreibung

Allgemeine Beschreibung

Studies report that MDGA1 is a layer-specific marker and an area-specific marker, being expressed in layers 2/3 throughout the neocortex, but within the primary somatosensory area (S1), MDGA1 is also uniquely expressed in layers 4 and 6a. Comparisons with other markers, including cadherins, serotonin, cytochrome oxidase, RORß, and COUP-TF1, reveal unique features of patterned expression of MDGA1 within cortex and S1 barrels. Furthermore, during early stages of development, MDGA1 is expressed by Reelin- and Tbr1-positive Cajal–Retzius neurons that originate from multiple sources outside of neocortex and emigrate into it. At even earlier stages, MDGA1 is expressed by the earliest diencephalic and mesencephalic neurons, which appear to migrate from a MDGA1-positive domain of progenitors in the diencephalon and form a "preplate." These findings show that MDGA1 is a unique marker for studies of cortical lamination and area patterning and together with recent reports suggest that MDGA1 has critical functions in forebrain/midbrain development.

Spezifität

This antibody recognizes the MAM domain of MDGA1.

Immunogen

Epitope: MAM domain

KLH-conjugated linear peptide corresponding to the MAM domain of MDGA1.

Anwendung

Research Category
Neurowissenschaft

Research Sub Category
Entwicklungsneurowissenschaft

Anti-MDGA1 Antibody detects level of MDGA1 & has been published & validated for use in Western Blotting.

Qualität

Evaluated by Western Blot in untreated and PNGase F treated mouse P7 brain tissue lysate.

Western Blot Analysis: 0.5 µg/mL of this antibody detected MDGA1 in 5 µg of untreated and PNGase F treated mouse P7 brain tissue lysate. This antibody recognizes glycosolated (>160 kDa) (lane 1) and deglycosolated MDGA1 (lane 2).

Zielbeschreibung

~130kDa observed. UniProt describes 2 isoforms produced by alternative splicing at ~106 kDa (Isoform 1), ~107 kDa (Isoform 2). An uncharacterized band may be observed at ~48 kDa in some tissue lysates.

Physikalische Form

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Lagerung und Haltbarkeit

Stable for 1 year at 2-8°C from date of receipt.

Hinweis zur Analyse

Control
Untreated and PNGase F treated mouse P7 brain tissue lysate.

Sonstige Hinweise

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklassenschlüssel: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable