AB1840
Anti-Interleukin-8 Antibody
serum, Chemicon®
Synonym(e):
IL-8
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Alle Fotos(1)
About This Item
UNSPSC-Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Biologische Quelle
rabbit
Qualitätsniveau
Antikörperform
serum
Antikörper-Produkttyp
primary antibodies
Klon
polyclonal
Speziesreaktivität
sheep
Hersteller/Markenname
Chemicon®
Methode(n)
ELISA: suitable
NCBI-Hinterlegungsnummer
UniProt-Hinterlegungsnummer
Versandbedingung
dry ice
Posttranslationale Modifikation Target
unmodified
Angaben zum Gen
sheep ... Cxcl8(443418)
Spezifität
Reacts with ovine IL-8. No reactivity detected to recombinant ovine IL-1beta, IL-6, MCP or TNFalpha.
Immunogen
Recombinant ovine IL-8
Anwendung
Research Category
Entzündung & Immunologie
Entzündung & Immunologie
Research Sub Category
Cytokine & Cytokin-Rezeptoren
Cytokine & Cytokin-Rezeptoren
Anti-Interleukin-8 Antibody detects level of Interleukin-8 & has been published & validated for use in ELISA.
Detecting antibody in ELISA, using monoclonal anti-ovine IL-8 as capture antibody. Recommended dilution factor 1:500. See suggested protocol.
Optimal working dilutions must be determined by the end user.
Suggested ELISA Protocol For Ovine IL-8
1. Coat high capacity microtiter wells (e.g. Nunc maxisorp) with monoclonal anti-IL-8 antibody (Chemicon Catalog Number MAB1044) at a concentration of 5 μg/mL in 0.1 M carbonate buffer pH 9.6 overnight at 4ºC.
2. Wash wells 3 x with PBS + 0.05% Tween 20 (PBST).
3. Add samples to wells. Incubate at room temperature in a humidified box for 1 hour. Wash 3 x with PBST.
4. Dilute rabbit anti-IL-8 (Chemicon Catalog Number AB1840) 1:500 in PBST and add 100 μL to wells. Incubate at room temperature for 30 minutes. Wash 3 x with PBST.
5. Dilute anti-rabbit IgG-HRP to appropriate level in PBST and add 100 μL to wells. Incubate 30 minutes at room temperature. Wash 3 x with PBST.
6. Add 100 μL of TMB chromagen to each well. Incubate for 5-10 minutes and stop reaction with 50 μL of 2M sulfuric acid.
Notes:
The anti-rabbit IgG HRP must be adsorbed against mouse immunoglobulin, and should be checked for non-reactivity with coated plates.
Non-specific binding may be reduced by addition of protein such as skim milk powder to the diluents.
Assay sensitivity may be altered by adjusting incubation times and reagent concentrations.
Optimal working dilutions must be determined by the end user.
Suggested ELISA Protocol For Ovine IL-8
1. Coat high capacity microtiter wells (e.g. Nunc maxisorp) with monoclonal anti-IL-8 antibody (Chemicon Catalog Number MAB1044) at a concentration of 5 μg/mL in 0.1 M carbonate buffer pH 9.6 overnight at 4ºC.
2. Wash wells 3 x with PBS + 0.05% Tween 20 (PBST).
3. Add samples to wells. Incubate at room temperature in a humidified box for 1 hour. Wash 3 x with PBST.
4. Dilute rabbit anti-IL-8 (Chemicon Catalog Number AB1840) 1:500 in PBST and add 100 μL to wells. Incubate at room temperature for 30 minutes. Wash 3 x with PBST.
5. Dilute anti-rabbit IgG-HRP to appropriate level in PBST and add 100 μL to wells. Incubate 30 minutes at room temperature. Wash 3 x with PBST.
6. Add 100 μL of TMB chromagen to each well. Incubate for 5-10 minutes and stop reaction with 50 μL of 2M sulfuric acid.
Notes:
The anti-rabbit IgG HRP must be adsorbed against mouse immunoglobulin, and should be checked for non-reactivity with coated plates.
Non-specific binding may be reduced by addition of protein such as skim milk powder to the diluents.
Assay sensitivity may be altered by adjusting incubation times and reagent concentrations.
Physikalische Form
Rabbit serum. Liquid containing 0.1% sodium azide.
Lagerung und Haltbarkeit
Maintain at -20°C in undiluted aliquots for up to 12 months. Avoid repeated freeze/thaw cycles.
Rechtliche Hinweise
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Haftungsausschluss
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Lagerklassenschlüssel
10 - Combustible liquids
WGK
WGK 1
Analysenzertifikate (COA)
Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.
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Tate Gisslen et al.
Innate immunity, 20(2), 214-224 (2013-06-12)
Histologic chorioamnionitis, frequently associated with preterm births and adverse outcomes, results in prolonged exposure of preterm fetuses to infectious agents and pro-inflammatory mediators, such as LPS. Endotoxin tolerance-type effects were demonstrated in fetal sheep following repetitive systemic or intra-amniotic (i.a.)
Modulation of lipopolysaccharide-induced chorioamnionitis in fetal sheep by maternal betamethasone.
Wolfe, KB; Snyder, CC; Gisslen, T; Kemp, MW; Newnham, JP; Kramer, BW; Jobe, AH; Kallapur, S
Reproductive Sciences null
Chronic fetal exposure to Ureaplasma parvum suppresses innate immune responses in sheep.
Kallapur, SG; Kramer, BW; Knox, CL; Berry, CA; Collins, JJ; Kemp, MW; Nitsos, I; Polglase et al.
Journal of immunology (Baltimore, Md. : 1950) (1950)
Selective exposure of the fetal lung and skin/amnion (but not gastro-intestinal tract) to LPS elicits acute systemic inflammation in fetal sheep.
Kemp, MW; Kannan, PS; Saito, M; Newnham, JP; Cox, T; Jobe, AH; Kramer, BW; Kallapur, SG
Testing null
Charlotte van Gorp et al.
Nutrients, 11(5) (2019-05-01)
Chorioamnionitis, clinically most frequently associated with Ureaplasma, is linked to intestinal inflammation and subsequent gut injury. No treatment is available to prevent chorioamnionitis-driven adverse intestinal outcomes. Evidence is increasing that plant sterols possess immune-modulatory properties. Therefore, we investigated the potential
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