70953
Rosetta Competent Cells - Novagen
Rosetta host strains are BL21 derivatives designed to enhance the expression of eukaryotic proteins that contain codons rarely used in E. coli.
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About This Item
UNSPSC-Code:
41106202
Empfohlene Produkte
Biologische Quelle
Escherichia coli
Qualitätsniveau
Hersteller/Markenname
Novagen®
Lagerbedingungen
OK to freeze
Wachstumsmodus
adherent or suspension
Morphologie
rod shaped
Methode(n)
microbiological culture: suitable
Zelltransformation
transformation efficiency: >2×106 cfu/μg
Versandbedingung
dry ice
Lagertemp.
−70°C
Verwandte Kategorien
Allgemeine Beschreibung
Genotype: F-ompT hsdSB(rB- mB-) gal dcm pRARE (CamR)
This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges us to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.
This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges us to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.
Rosetta host strains are BL21 derivatives designed to enhance the expression of eukaryotic proteins that contain codons rarely used in E. coli. These strains supply tRNAs for AGG, AGA, AUA, CUA, CCC, GGA codons on a compatible chloramphenicol-resistant plasmid. Thus the Rosetta strains provide for “universal” translation which is otherwise limited by the codon usage of E. coli. The tRNA genes are driven by their native promoters.
Rosetta host strains are BL21 derivatives designed to enhance the expression of eukaryotic proteins that contain codons rarely used in E. coli.
Komponenten
0.4 ml1 mlComponent
•2 × 0.2 ml5 × 0.2 mlRosetta Competent Cells
•2 × 2 ml4 × 2 mlSOC Medium
•10 µl10 µlTest Plasmid
•2 × 0.2 ml5 × 0.2 mlRosetta Competent Cells
•2 × 2 ml4 × 2 mlSOC Medium
•10 µl10 µlTest Plasmid
Warnhinweis
Toxicity: Multiple Toxicity Values, refer to MSDS (O)
Rechtliche Hinweise
NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany
Lagerklassenschlüssel
10 - Combustible liquids
WGK
WGK 2
Analysenzertifikate (COA)
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