539568

PP2B, Human, Recombinant, E. coli

• Synonym(e): CaN, Protein Phosphatase 2B, Calcineurin
• UNSPSC-Code: 12352202
• NACRES: NA.77

Eigenschaften

• Form: liquid
• Qualitätsniveau: 100
• Spezifische Aktivität: ≥300,000 U/mg; ≥50,000 units/mL
• Hersteller/Markenname: Calbiochem^®
• Lagerbedingungen: OK to freeze; avoid repeated freeze/thaw cycles
• Versandbedingung: wet ice
• Lagertemp.: −70°C

Beschreibung

Allgemeine Beschreibung

Recombinant, human calcineurin Aα (catalytic subunit) and calcineurin B (regulatory subunit) co-expressed with yeast myristoyl-CoA:protein N-myristoyltransferase. A Ca²⁺/CaM-dependent protein phosphatase. The resulting highly active calcineurin is N-myristoylated on the CaN B-subunit, as is the native protein. M.W. 60,000 (A subunit) and 15,000 (B subunit).

Warnhinweis

Toxicity: Standard Handling (A)

Einheitendefinition

One unit is defined as the amount of enzyme that will release 1.0 pmol of phosphate from the RII phosphopeptide substrate (DLDVPIPGRFDRRVpSVAAE; Cat. No. 207008) in the presence of calmodulin per min at 30°C, pH 7.4.

Physikalische Form

In 100 mM NaCl, 50 mM Tris, 6 mM MgCl₂, 5 mM DTT, 0.5 mM CaCl₂, 0.025% NP-40.

Rekonstituierung

Following initial thaw, aliquot and freeze (-70°C). Please refer to vial label for lot-specific reconstitution volume.

Hinweis zur Analyse

Two distinct bands by SDS-PAGE

Sonstige Hinweise

Guerini, D. 1997. Biochem. Biophys. Res. Commun. 235, 271.
Mondragon, A., et al. 1997. Biochemistry 36, 4934.
Wei, Q. and Lee, E.Y. 1997. Biochemistry36, 7418.

Rechtliche Hinweise

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Sicherheitshinweise

• Lagerklassenschlüssel: 10 - Combustible liquids
• WGK: WGK 1
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable