5.06029

LipidGreen2

• Synonym(e): LipidGreen2
• Empirische Formel (Hill-System): C₂₂H₂₉NO₄
• Molekulargewicht: 371.47
• UNSPSC-Code: 12352202
• NACRES: NA.21

Eigenschaften

• Assay: ≥97% (HPLC)
• Qualitätsniveau: 100
• Form: oil
• Hersteller/Markenname: Calbiochem^®
• Lagerbedingungen: OK to freeze; protect from light
• Farbe: orange-yellow
• Löslichkeit: DMSO: 100 mg/mL
• Lagertemp.: 2-8°C

Beschreibung

Allgemeine Beschreibung

A cell-permeable N-dimethylallylated 3-hydroxyindole based compound that is shown to selectively stain neutral lipids, but not phospholipids, in various cells lines with bright fluorescence and with minimal non-specific background. Generates stronger staining with lipid droplets (~10 µM) when compared to Nile Red. Shown to be more effective than BODIPY^® 493/503 in assessing hepatotoxicity of several known compounds using HepG2 cells. Also suitable for staining fat deposits in developing zebrafish embryos. Can be used in conjunction with LipidToxt red dye for multicolor imaging of neutral lipids.

A cell-permeable N-dimethylallylated 3-hydroxyindole based compound that is shown to selectively stain neutral lipids, but not phospholipids, in various cells lines with bright fluorescence and with minimal non-specific background. Generates stronger staining with lipid droplets (~10 µM) when compared to Nile Red. Shown to be more effective than BODIPY^® 493/503 in assessing hepatotoxicity of several known compounds using HepG2 cells. Also suitable for staining fat deposits in developing zebrafish embryos. Can be used in conjunction with LipidToxt red dye for multicolor imaging of neutral lipids.

Please note that the molecular weight for this compound is batch-specific due to variable water content.

Biochem./physiol. Wirkung

Cell permeable: yes

Verpackung

Packaged under inert gas

Warnhinweis

Toxicity: Standard Handling (A)

Rekonstituierung

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 1 month at -20°C.

Sonstige Hinweise

Chun, H., et al, 2013, Mol Biosys.9, 630.

Rechtliche Hinweise

BODIPY is a registered trademark of Life Technologies

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Sicherheitshinweise

• Lagerklassenschlüssel: 10 - Combustible liquids
• WGK: WGK 3
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable