444905

Anti-Mast Cell Tryptase Mouse mAb (AA1)

liquid, clone AA1, Calbiochem^®

• UNSPSC-Code: 12352203
• NACRES: NA.41

Eigenschaften

• Biologische Quelle: mouse
• Qualitätsniveau: 100
• Antikörperform: purified antibody
• Antikörper-Produkttyp: primary antibodies
• Klon: AA1, monoclonal
• Form: liquid
• Enthält nicht: preservative
• Speziesreaktivität: monkey, canine, human, feline
• Hersteller/Markenname: Calbiochem^®
• Lagerbedingungen: OK to freeze; avoid repeated freeze/thaw cycles
• Isotyp: IgG1
• Versandbedingung: wet ice
• Lagertemp.: −20°C
• Posttranslationale Modifikation Target: unmodified
• Angaben zum Gen: human ... TPSAB1(7177)

Beschreibung

Allgemeine Beschreibung

Protein G purified mouse monoclonal antibody. Recognizes the ~32-38 kDa α- and β-isoforms of tryptase in mast cells.

Recognizes the 32-38 kDa α- and β-isoforms of tryptase in mast cells.

This Anti-Mast Cell Tryptase Mouse mAb (AA1) is validated for use in Frozen Sections, Immunoblotting, Paraffin Sections for the detection of Mast Cell Tryptase.

Immunogen

Human

native, human mast cell, tryptase

Anwendung

Frozen Sections (0.06-0.12 µg/ml)

Immunoblotting (1 µg/ml)

Paraffin Sections (0.06-0.12 µg/ml, heat pre-treatment required, see comments)

Verpackung

Please refer to vial label for lot-specific concentration.

Warnhinweis

Toxicity: Standard Handling (A)

Physikalische Form

In 10 mM PBS, pH 7.4.

Rekonstituierung

Following initial thaw, aliquot and freeze (-20°C).

Hinweis zur Analyse

Positive Control
Cat, dog, human, or monkey mast cells

Sonstige Hinweise

Sommerhoff, C.P., et al. 2000. Biochim. Biophys. Acta.1477, 75.
Little, S.S., and Johnson, D.A. 1995. Biochem. J.307, 341.
Morgan, S.J., et al. 1991. J. Allergy Clin. Immunol.87, 111.
Walls, A.F., et al. 1990. J. Pathol.162, 119.
Wall, A.F., et al. 1990. Clin. Exp. Allergy20, 581.

Staining of formalin-fixed paraffin sections requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at room temperature for 20 min. Variables associated with assay conditions will dictate the optimal working dilution.

Rechtliche Hinweise

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Sicherheitshinweise

• Lagerklassenschlüssel: 10 - Combustible liquids
• WGK: WGK 1
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable