422426

KGF/FGF-7, Mouse, Recombinant

• UNSPSC-Code: 12352202
• NACRES: NA.77

Eigenschaften

• Assay: ≥90% (SDS-PAGE)
• Qualitätsniveau: 100
• Form: solid
• Hersteller/Markenname: Calbiochem^®
• Lagerbedingungen: OK to freeze
• Verunreinigungen: <1.0 EU/μg protein Endotoxin
• Versandbedingung: ambient
• Lagertemp.: −20°C

Beschreibung

Allgemeine Beschreibung

Recombinant, mouse keratinocyte growth factor (KGF) consisting of amino acids 32-194 and expressed in E. coli. KGF plays an essential role in development, tumorigenesis, angiogenesis, morphogenesis, and wound healing. Mature mouse KGF amino acid sequence is 96% identical with rat, bovine, ovine, equine, and human forms and is active across species.

Biochem./physiol. Wirkung

ED₅₀ = 10-50 ng/ml as measured in a cell proliferation assay using 4MBr-5 monkey epithelial cells.

Warnhinweis

Toxicity: Standard Handling (A)

Physikalische Form

Lyophilized from 0.2 µm sterile-filtered MOPS, Na₂SO₄, EDTA, DTT BSA.

Rekonstituierung

Following reconstitution refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C) for long-term storage. Stock solutions are stable for up to 1 month at 4°C or for up to 3 months at -20°C.

Reconstitute to 250 µg/ml in sterile PBS containing at least 0.1% human or bovine serum albumin.

Sonstige Hinweise

Belleudi, F., et al. 2007. Traffic8, 1854.
Finch, P.W. and J.S. Rubin. 2006. J. Natl. Cancer Inst.98, 812.
de Georgi, V., et al. 2007. Dermatol. Clin.25, 477.
Niu, J., et al. 2007. J. Biol. Chem.282, 6001.
Werner, S., et al. 2007. J. Invest. Dermatol.127, 998.
Cardinali, G., et al. 2005. J. Invest. Dermatol.125, 1190.
Geer, D.J., et al. 2005. Am. J. Pathol.167, 1575.
Hsu, YR., et al. 1999. Biochemistry38, 2523.
Qiao, J., et al. 1999. Development126, 547.
Werner, S., 1998. Cytokine Growth Factor Rev.9, 153.
Guo, L., et al. 1996. Genes Dev.10, 165.
Mason, I.J., et al. 1994. Mech. Dev.45, 15.

Rechtliche Hinweise

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Sicherheitshinweise

• Lagerklassenschlüssel: 11 - Combustible Solids
• WGK: WGK 2