362180

N-Glycosidase A, Almond

Native N-Glycosidase A from almonds. Cleaves N-glycan chains from glycopeptides, including those with α1,3-linked core fucose. This enzyme cleaves between GlcNAc and asparagine. Note: 1 mU = 1 milliunit.

• Synonym(e): N-Glycosidase A, Almond
• CAS-Nummer: 83534-39-8
• EC-Nummer: 3.5.1.52 (BRENDA, IUBMB)
• MDL-Nummer: MFCD00131222
• UNSPSC-Code: 12352202

Eigenschaften

• Qualitätsniveau: 100
• Form: liquid
• Spezifische Aktivität: ≥500 mU/mg protein
• Hersteller/Markenname: Calbiochem^®
• Lagerbedingungen: OK to freeze
• Fremdaktivität: α- and β-galactosidase, β-glucosidase ≤0.1%; proteases, none detected
• Versandbedingung: wet ice
• Lagertemp.: −20°C

Beschreibung

Allgemeine Beschreibung

Native N-Glycosidase A from almonds. Cleaves N-glycan chains from glycopeptides, including those with α1,3-linked core fucose. This enzyme cleaves between GlcNAc and asparagine.

Native N-Glycosidase A from almonds. Cleaves N-glycan chains from glycopeptides, including those with α1,3-linked core fucose. This enzyme cleaves between GlcNAc and asparagine. Has an optimal pH of 4.0-6.0.

Native N-Glycosidase A from almonds. Cleaves N-glycan chains from glycopeptides, including those with α1,3-linked core fucose. This enzyme cleaves between GlcNAc and asparagine. Note: 1 mU = 1 milliunit.

Note: 1 mU = 1 milliunit.

Warnhinweis

Toxicity: Standard Handling (A)

Einheitendefinition

One unit is defined as the amount of enzyme that will catalyze the release of 1.0 µmol hybrid and high-mannose oligosaccharides from ovalbumin glycopeptide per min at 37°C, pH 5.0.

Physikalische Form

In 50 mM citrate/phosphate buffer, 50% glycerol, pH 5.0.

Rekonstituierung

Following initial thaw, aliquot and freeze (-20°C).

Sonstige Hinweise

Means, R.E. and Desrosiers, R.C. 2000. J. Virol.74, 11181.
Fan, J.Q., and Lee, Y.C. 1997. J. Biol. Chem. 272, 27058.
Altmann, F., et al. 1995. Glycoconj. J. 12, 84.
Takahashi, N., and Nishibe, H. 1978. J. Biochem. 84, 1467.

Rechtliche Hinweise

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Sicherheitshinweise

• Lagerklassenschlüssel: 10 - Combustible liquids
• WGK: WGK 2