251520-M

DAF-FM DA

A cell-permeable, photo-stable nitric oxide (NO) fluorescent indicator with a detection limit of ~3 nM.

• Synonym(e): DAF-FM DA, 3-Amino, 4-aminomethyl-2ʹ,7ʹ-difluorofluorescein Diacetate
• Empirische Formel (Hill-System): C₂₅H₁₈F₂N₂O₇
• CAS-Nummer: 254109-22-3
• Molekulargewicht: 496.42
• MDL-Nummer: MFCD16872050
• UNSPSC-Code: 12352204
• NACRES: NA.25

Eigenschaften

• Qualitätsniveau: 100
• Assay: ≥98% (HPLC)
• Form: solution
• Hersteller/Markenname: Calbiochem^®
• Lagerbedingungen: OK to freeze; protect from light
• Farbe: pale yellow
• Fluoreszenz: λ_ex ~500 nm; λ_em ~515 nm
• Lagertemp.: −20°C
• InChI: 1S/C25H18F2N2O7/c1-10(30)33-20-8-18-13(6-15(20)26)25(12-4-5-17(29-3)23(28)22(12)24(32)36-25)14-7-16(27)21(34-11(2)31)9-19(14)35-18/h4-9,29H,28H2,1-3H3
• InChIKey: BEVHTVRRVVEMEF-UHFFFAOYSA-N

Beschreibung

Allgemeine Beschreibung

A cell-permeable, photo-stable nitric oxide (NO) fluorescent indicator with a detection limit of ~3 nM. Intracellularly releases DAF-FM (Cat. No. 251515) by the action of esterases. Reacts with NO, in the presence of O₂, resulting in a triazolo-fluorescein analog (DAF-FM T) that exhibits about 160-fold greater fluorescence quantum efficiency. The fluorescence is reported to be stable above pH 5.8.

A cell-permeable, photo-stable nitric oxide (NO) fluorescent indicator with a detection limit of ~3 nM. Releases DAF-FM (Cat. No. 251515) by the action of intracellular esterases. Reacts with NO, in the presence of oxygen, resulting in the formation of a triazolo-fluorescein analog (DAF-FM T) that exhibits about 160-fold greater fluorescence quantum efficiency. The fluorescence intensity is reported to be stable above pH 5.8. Excitation max.: ~500 nm; emission max.: ~515 nm.

Biochem./physiol. Wirkung

Primary Target
Nitric oxide (NO) fluorescent indicator

Warnhinweis

Toxicity: Irritant (B)

Physikalische Form

A 5 mM solution in DMSO.

Rekonstituierung

Following initial thaw, aliquot and freeze at (-20°C). Use immediately after dilution; discard any unused portion.

Sonstige Hinweise

Kojima, H., et al. 2001. J. Neurochem.76, 1404.
Park. J.M., et al. 2001. Br. J. Pharmacol.132, 1876.
Itoh, Y., et al. 2000. Anal. Biochem.287, 203.
Kojima, H., et al. 1999. Angew Chem. Int. Ed.38, 3209.

Rechtliche Hinweise

Manufactured by Daiichi Pure Chemicals Co., Ltd

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Sicherheitshinweise

• Lagerklassenschlüssel: 10 - Combustible liquids
• WGK: WGK 2
• Flammpunkt (°F): 188.6 °F - closed cup - (Dimethylsulfoxide)
• Flammpunkt (°C): 87 °C - closed cup - (Dimethylsulfoxide)