17-286
Cdc42 Activation Assay Kit
Non-radioactive cdc42 Activation Assay Kit can be used to precipitate cdc42-GTP from cell lysates subjected to various treatments & detection by a cdc42 specific monoclonal antibody.
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About This Item
UNSPSC-Code:
12161503
eCl@ss:
32161000
NACRES:
NA.84
Empfohlene Produkte
Qualitätsniveau
Hersteller/Markenname
Upstate®
Methode(n)
activity assay: suitable
affinity binding assay: suitable
NCBI-Hinterlegungsnummer
UniProt-Hinterlegungsnummer
Nachweisverfahren
chemiluminescent
Versandbedingung
wet ice
Angaben zum Gen
human ... CDC42(998)
Verpackung
Kit capacity: 30 assays
Komponenten
Rac/cdc42 Assay Reagent (PAK1 PBD, agarose) (Cat.# 14-325)
Mg2+ Lysis/Wash Buffer, 5X (Cat.# 20-168)
100X GTPγS, 10mM (Cat.# 20-176)
100X GDP, 100mM (Cat.# 20-177)
Anti-cdc42
Mg2+ Lysis/Wash Buffer, 5X (Cat.# 20-168)
100X GTPγS, 10mM (Cat.# 20-176)
100X GDP, 100mM (Cat.# 20-177)
Anti-cdc42
Qualität
Routinely evaluated by immunoprecipitating cdc42-GTP from 3T3/NIH cell lysate that has been loaded in vitro with GTPγS. Subsequent detection in western immunoblot using anti-cdc42.
Verlinkung
Replaces: SGT430
Rechtliche Hinweise
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Haftungsausschluss
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Signalwort
Danger
H-Sätze
Gefahreneinstufungen
Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1
Lagerklassenschlüssel
10 - Combustible liquids
Analysenzertifikate (COA)
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Isao Usui et al.
The Journal of biological chemistry, 278(16), 13765-13774 (2003-02-05)
We investigated the role of cdc42, a Rho GTPase family member, in insulin-induced glucose transport in 3T3-L1 adipocytes. Microinjection of anti-cdc42 antibody or cdc42 siRNA led to decreased insulin-induced and constitutively active G(q) (CA-G(q); Q209L)-induced GLUT4 translocation. Adenovirus-mediated expression of
Distinct mechanisms of clathrin-independent endocytosis have unique sphingolipid requirements.
Zhi-Jie Cheng, Raman Deep Singh, Deepak K Sharma, Eileen L Holicky, Kentaro Hanada et al.
Molecular Biology of the Cell null
V Benard et al.
The Journal of biological chemistry, 274(19), 13198-13204 (1999-05-01)
A major function of Rac2 in neutrophils is the regulation of oxidant production important in bacterial killing. Rac and the related GTPase Cdc42 also regulate the dynamics of the actin cytoskeleton, necessary for leukocyte chemotaxis and phagocytosis of microorganisms. Although
S J Taylor et al.
Current biology : CB, 6(12), 1621-1627 (1996-12-01)
Ras proteins play an essential role in the transduction of signals from a wide range of cell-surface receptors to the nucleus. These signals may promote cellular proliferation or differentiation, depending on the cell background. It is well established that Ras
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