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Merck

1.52258

Supelco

Chromolith® WP 300 Protein A (monolithic) HPLC Columns

L × I.D. 25 mm x 4.6 mm HPLC Column

Synonym(e):

Chromolith® WP 300 Protein A 25-4.6 HPLC column

Anmeldenzur Ansicht organisationsspezifischer und vertraglich vereinbarter Preise


About This Item

UNSPSC-Code:
41115700
NACRES:
SB.52

product name

Chromolith® WP 300 Protein A, L × I.D. 25 mm x 4.6 mm HPLC column

Materialien

PEEK column

Produktlinie

Chromolith®

Leistungsmerkmale

endcapped: no
endcapped: no

Verpackung

1 ea of

Parameter

2 - 45 °C temp. range
2-45 °C temp. range
200 bar pressure
200 bar pressure

Methode(n)

HPLC: suitable
HPLC: suitable
LC/MS: suitable

Säule L × ID

2.5 cm × 4.6 mm

Oberflächenbereich

120 m2/g

Matrix

monolithic silica
monolithic silica

Aktive Matrixgruppe

Protein A bonding phase
Protein A bonding phase

Porengröße

2 μm macroporosity
2 μm macroporosity
30 nm mesoporosity
30 nm mesoporosity

operating pH range

1.5-7.5

Betriebs-pH-Wert

1.5-7.5

Trenntechnik

affinity

Lagertemp.

2-8°C
2-8°C

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Allgemeine Beschreibung

Affinity chromatography is a selective technique which takes advantage of specific molecular interactions, for example antigen and antibody. The Chromolith® WP 300 Protein A HPLC column is designed to monitor monoclonal antibody titer and yield determination from cell-culture supernatants. This analytical scale procedure helps to optimize the titer of monoclonal antibody for the optimal time for harvest of the monoclonal antibody products.

Chromolith® WP 300 Protein A 25-4.6 columns are made of monolithic silica with a characteristic bimodal pore structure.

Key Benefits:
—Rapid and accurate titer determination across a wide range of injection volumes
—Consistent binding efficiencies even at high flow rates
—Expanded column lifetimes

Learn more:
Brochure: Race through your Separations on Any System. Chromolith® Monolithic Silica HPLC Columns

Chromolith® WP 300 Protein A is available in 2 mm I.D. providing improved efficiency, sensitivity, and enhanced MS-compatibility.
Flyer: Rapid and Robust Antibody Quantitation - Chromolith® WP 300 Protein A HPLC columns

All Supelco HPLC Columns, including monolithic Chromolith® columns, are fully compatible to all HPLC and UHPLC Instruments.
Supelco HPLC Products: The Best Choice for Any LC Instrument

Rechtliche Hinweise

Chromolith is a registered trademark of Merck KGaA, Darmstadt, Germany

Analysenzertifikate (COA)

Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.

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Artikel

Silica monoliths with larger mesopores are a powerful tool for the fast separation of biomolecules including mAbs. The immobilization of protein A on epoxy-modified widepore (WP) silica rods enabled highly reproducible and fast, one minute, separations of mAbs. The Chromolith® WP 300 is also available with a protein A phase.

Verwandter Inhalt

HPLC separates and identifies large biomolecules like proteins and peptides by pumping the sample through a sorbent-packed column.

Analytical Chromolith® HPLC columns for Bio applications, 300A mesopores: RP-18, RP-8, RP-4, Prot-A, Epoxy

Unser Team von Wissenschaftlern verfügt über Erfahrung in allen Forschungsbereichen einschließlich Life Science, Materialwissenschaften, chemischer Synthese, Chromatographie, Analytik und vielen mehr..

Setzen Sie sich mit dem technischen Dienst in Verbindung.