1.25062

Fractogel^® EMD COO^- (M)

MiniChrom Fractogel^® COO (M), volume 1 mL, prepacked column

• Synonym(e): MiniChrom Column Fractogel^® COO (M), 1 ml
• UNSPSC-Code: 41115711

Eigenschaften

• ligand: (Carboxyethyl )
• Materialien: polypropylene housing
• Produktlinie: Fractogel^®
• Form: prepacked column
• Parameter: ≤8 bar pressure; ~300 cm/hr max. flow rate
• column I.D. × L: 8 mm × 20 mm
• Volumen: 1 mL
• Aktive Matrixgruppe: Methacrylate
• Mittlere Partikelgröße: 40-90 μm
• Kapazität: ~100 mL, resin binding capacity
• Anwendung(en): mAb; recombinant protein; vaccine development
• Trenntechnik: cation exchange
• Lagertemp.: 15-25°C

Beschreibung

Allgemeine Beschreibung

8 x 20mm

Leistungsmerkmale und Vorteile

MiniChrom Column Fractogel^® COO (M), 1 ml enables:

• Good resolution between monomers and aggregates
• Efficient aggregate and HCP removal combined with high yield
• Compatibility with 2.5 % (v/v) aqueous benzyl alcohol containing 150 mM NaCl storage solution

Due to the titration behavior, the ion exchange capacity can be used from pH 6 to pH 12. The separation of proteins is based on reversible electrostatic interactions between the negatively charged regions of the proteins′ surface and the support. Proteins are retained efficiently on Fractogel^® EMD COO^- (M) when the pH of the buffer is about 1 unit below their isoelectric points (pl).

The strength of the binding depends on the following:

• the buffer system
• pH value of the buffer which determines the surface charge of the protein
• the degree of the ionization of the functional groups of the exchanger
• the concentration of the counter ions
• the charge density on the support (protein binding capacity)

Hinweis zur Analyse

Aussehen: entspricht

Rechtliche Hinweise

FRACTOGEL is a registered trademark of Merck KGaA, Darmstadt, Germany