07-260

Anti-AUF1 Antibody

Upstate^®, from rabbit

• Synonym(e): Anti-AUF1, Anti-AUF1A, Anti-HNRPD, Anti-P37, Anti-hnRNPD0
• UNSPSC-Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Eigenschaften

• Biologische Quelle: rabbit
• Qualitätsniveau: 100
• Antikörperform: purified immunoglobulin
• Antikörper-Produkttyp: primary antibodies
• Klon: polyclonal
• Speziesreaktivität: human, mouse, rat
• Hersteller/Markenname: Upstate^®
• Methode(n): western blot: suitable
• Isotyp: IgG
• NCBI-Hinterlegungsnummer: NM_031370
• UniProt-Hinterlegungsnummer: Q14103
• Versandbedingung: dry ice
• Posttranslationale Modifikation Target: unmodified
• Angaben zum Gen: human ... HNRNPD(3184) ; mouse ... Hnrnpd(11991) ; rat ... Hnrnpd(79256)

Beschreibung

Allgemeine Beschreibung

A+U Rich RNA Binding Factor (AUF1) is comprised of four isoforms of 37, 40, 42, and 45 kDa. Although the role of each isoform has yet to be fully characterized, a direct correlation has been observed between each AUF1 isoform’s binding affinity and its RNA destabilizing activity toward different AREs (A + U rich element in the 3’ untranslated region), with the isoforms p37 and p42 being the most effective.

AUF1 is a bcl2 mRNA binding protein and potentially all its isoforms are able to form complexes with the bcl2 ARE. ARE mediated bcl2 mRNA downregulation during apoptosis involves AUF1 and suggest different roles for its four isoforms.

Spezifität

Recognizes AUF1 isoforms.

Immunogen

Purified AUF1

Anwendung

Research Category
Epigenetik & nukleäre Funktionen

Research Sub Category
Transkriptionsfaktoren

RNA-Bindeproteine (RBP)

Anti-AUF1 Antibody is a high quality Rabbit Polyclonal Antibody for the detection of AUF1 & has been validated in WB.

Qualität

routinely evaluated by immunoblot on RIPA lysates from HeLa nuclear extract or A431 cells

Zielbeschreibung

37, 40, 42, 45 kDa

Physikalische Form

Protein A purified

Format: Purified

Protein A Purified immunoglobulin in 30% glycerol, 0.07M Tris-glycine, pH 7.4, 0.105 M NaCl, 0.035% sodium azide as a preservative.

Lagerung und Haltbarkeit

Maintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Hinweis zur Analyse

Control
Positive Antigen Control: Catalog #12-309, Hela cell nuclear extract. Add an equal volume of Laemmli reducing sample buffer to 10 μL of extract and boil for 5 minutes to reduce the preparation. Load 20 μg of reduced extract per lane for minigels.

Sonstige Hinweise

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Rechtliche Hinweise

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklassenschlüssel: 10 - Combustible liquids
• WGK: WGK 1