06-1358

Anti-acetyl (Lys34) phospho (Ser36) Histone H1 Antibody

from rabbit, purified by affinity chromatography

• Synonym(e): Histone H1.4, Histone H1b
• UNSPSC-Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Eigenschaften

• Biologische Quelle: rabbit
• Qualitätsniveau: 100
• Antikörperform: affinity isolated antibody
• Antikörper-Produkttyp: primary antibodies
• Klon: polyclonal
• Aufgereinigt durch: affinity chromatography
• Speziesreaktivität: human, bovine
• Speziesreaktivität (Voraussage durch Homologie): canine (based on 100% sequence homology), rhesus macaque (based on 100% sequence homology)
• Methode(n): dot blot: suitable; western blot: suitable
• NCBI-Hinterlegungsnummer: NP_005312
• UniProt-Hinterlegungsnummer: P10412
• Versandbedingung: wet ice
• Posttranslationale Modifikation Target: phosphorylation (pSer36), acetylation (Lys34)
• Angaben zum Gen: bovine ... H1-4(617854) ; dog ... H1-4(488269) ; human ... H1-4(3008) ; rhesus monkey ... H1-4(696872)

Beschreibung

Allgemeine Beschreibung

H1.4 is one of four variants of histone H1 (H1.2-H1.5) that is found in somatic cells. The H1 histones may function as linker proteins binding DNA via their GH domain, and enabling the wrapping of DNA around the core nucleosomal structure formed from the association of H2A, H2B, H3, and H4. In response to various stimuli, histones undergo a number of post-translational modifications (PTM) including acetylation, phoshorylation, and methylation. Previous studies have suggested that H1.4 may be phosphorylated during mitosis and interphase resulting in increased transcription of target genes by RNA polymerase I and II, and therefore this PTM on H1.4 may have implications for cell growth. H1.4 may also undergo acetylation of its N-terminal lysine residues. Often, it is the pattern of different modifications on several histone residues that alter chromatin and affect gene expression, rather than modification of single residues.

Spezifität

Other homologies: Mouse and Rat (90% sequence homology).

This antibody recognizes Histone H1 acetylated at Lys34 and phosphorylated at Ser36.

Immunogen

Epitope: Acetylated Lys34 and phosphorylated Ser36

KLH-conjugated linear peptide corresponding to human Histone H1 acetylated at Lys34 and phosphorylated at Ser36.

Anwendung

Research Category
Epigenetik & nukleäre Funktionen

Research Sub Category
Histone

Dot Blot Specificity Analysis: Unmodified and various modified Histone peptides (see Table) were probed with acetyl (Lys34) phospho (Ser36) Histone H1.

Use Anti-acetyl (Lys34) phospho (Ser36) Histone H1 Antibody (Rabbit Polyclonal Antibody) validated in WB & DB to detect acetyl (Lys34) phospho (Ser36) Histone H1 also known as Histone H1b.

Qualität

Evaluated by Western Blot in calf thymus histone extract.

Western Blot Analysis: A 1:37 dilution of this antibody detected Histone H1 on 10 µg of calf thymus histone extract.

Zielbeschreibung

~34 kDa observed

Physikalische Form

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Lagerung und Haltbarkeit

Stable for 1 year at 2-8°C from date of receipt.

Hinweis zur Analyse

Control
Calf thymus histone extract

Sonstige Hinweise

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklassenschlüssel: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable