05-1059

Anti-phospho-p38α(Thr180/Tyr182) Antibody, clone 8.78.8, rabbit monoclonal

culture supernatant, clone 8.78.8, from rabbit

• Synonym(e): CSAID-binding protein, Csaids binding protein, Cytokine suppressive anti-inflammatory drug-binding protein, MAP kinase MXI2, MAP kinase p38 alpha, MAX-interacting protein 2, Mitogen-activated protein kinase p38 alpha, cytokine suppressive anti-inflammato
• UNSPSC-Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Eigenschaften

• Biologische Quelle: rabbit
• Qualitätsniveau: 100
• Antikörperform: culture supernatant
• Antikörper-Produkttyp: primary antibodies
• Klon: 8.78.8, monoclonal
• Speziesreaktivität: human, vertebrates, mouse
• Methode(n): immunofluorescence: suitable; western blot: suitable
• Isotyp: IgG
• NCBI-Hinterlegungsnummer: NP_001306
• UniProt-Hinterlegungsnummer: Q15759
• Versandbedingung: wet ice
• Posttranslationale Modifikation Target: phosphorylation (pThr180/pTyr182)
• Angaben zum Gen: human ... MAPK14(1432)

Beschreibung

Allgemeine Beschreibung

The p38 family refers to the SAPK2/3 Kinases (Stess-Activated Protein Kinases), a subfamily of JNK/SAPK family. p38/SAPK Kinases are also activated by stresses, most notably inflammatory cytokines, irradiation, and certain toxins such as anisomycin and arsenite. SAPKs have been implicated in many pathological conditions, including inflammation, cancer and neurodegenerative diseases. As with the other MAPK family members (ERK, JNK, p38MAPK, and ERK5), p38 is activated through phosphorylation on both threonine and tyrosine residues at the Thr-X-Tyr dual phosphorylation motif, where X is Gly for p38. Once phosphorylated and active, it then goes on to phosphorylate downstream targets in both the cytoplasm and the nucleus. Some of their main targets of p38 are the kinase MAPKAPK2 and the transcription factor ATF2 that are activated via their phosphorylation.

Spezifität

Predicted to cross-react with many other species based on 100% sequence homology with immunogen.

This antibody detects p38α when phosphorylated on Thr180/Tyr182.

Immunogen

Epitope: Phosphorylated Thr180/Tyr182

Synthetic peptide corresponding to amino acids surrounding and including phosphorylated Thr180/Tyr182 of human p38α.

Anwendung

Research Sub Category
MAP-Kinasen

Entzündungs- & Autoimmunmechanismen

Anti-phospho-p38α(Thr180/Tyr182) Antibody, clone 8.78.8 detects level of phospho-p38α(Thr180/Tyr182) & has been published & validated for use in WB & IF.

Qualität

Evaluated by western blot on anisomycin-treated HeLa cell lysate.
Western Blot Analysis: 1:5,000-1:10,000 dilution of this antibody was used to detect p38 phosphorylated on Thr180/Tyr182 in anisomycin-treated HeLa and NIH/3T3 cell lysate.

Zielbeschreibung

38 kDa

Physikalische Form

Rabbit monoclonal antibody in culture supernatant containing 0.05% sodium azide.

Sicherheitshinweise

• Lagerklassenschlüssel: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable