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SHC016

Sigma-Aldrich

MISSION® pLKO.1-puro Non-Target shRNA Control Plasmid DNA

Targets no known genes from any species

Sinónimos:

MISSION® Control Vectors, negative control, negative shRNA control, non-target control, non-target shRNA, non-target shRNA control, shRNA control

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About This Item

MDL number:
MFCD07785395
UNSPSC Code:
41106609
NACRES:
NA.51

Quality Level

200

product line

MISSION®

concentration

500 ng/μL in TE buffer; DNA (10μg of plasmid DNA)

shipped in

dry ice

storage temp.

−20°C

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General description

The MISSION pLKO.1-puro Non-Target shRNA Control Plasmid DNA is a lentivirus plasmid vector. The vector contains an shRNA insert that does not target any known genes from any species, making it useful as a negative control in experiments using the MISSION shRNA library clones. This allows one to examine the effect of transfection of a short-hairpin on gene expression and interpret the knockdown effect seen with shRNA clones. Ampicillin and puromycin antibiotic resistance genes provide selection in bacterial or mammalian cells respectively. In addition, self-inactivating replication incompetent viral particles can be produced in packaging cells (HEK293T) by co-transfection with compatible packaging plasmids. The Non-Target shRNA Control Plasmid DNA is provided as 10 μg of plasmid DNA in Tris-EDTA (TE) buffer at a concentration of 500 ng/μl.

Application

MISSION® pLKO.1-puro non-target shRNA control plasmid DNA has been used as a control during transduction:
  • in tumor cells for multicolour imaging
  • in human adult low calcium temperature keratinocytes
  • in mouse embryonic fibroblasts, to study the biological functions of IP6K1 (inositol hexakisphosphate kinase)
  • to study the function of Zac1 (zinc finger protein regulating apoptosis and cell cycle arrest) expression in astroglial differentiation
To see more application data, protocols, vector maps visit sigma.com/shrna.

Legal Information

Use of this product is subject to one or more license agreements. For details, please see http://sigmaaldrich.com/missionlicense.
MISSION is a registered trademark of Merck KGaA, Darmstadt, Germany

Optional

Referencia del producto
Descripción
Precios

SHC001

MISSION® pLKO.1-puro Empty Vector Control Plasmid DNA, Contains no shRNA insert

SHC002

MISSION® pLKO.1-puro Non-Mammalian shRNA Control Plasmid DNA, Targets no known mammalian genes

SHC003

MISSION® pLKO.1-puro-CMV-TurboGFP™ Positive Control Plasmid DNA, Green fluorescent protein marker to monitor transduction efficiency

SHC004

MISSION® pLKO.1-puro TurboGFP™ shRNA Control Plasmid DNA, shRNA sequence targeting tGFP

SHC005

MISSION® eGFP shRNA Control Vector, shRNA sequence targeting eGFP

SHC007

MISSION® Luciferase shRNA Control Vector, shRNA sequence targeting luciferase

SHC012

MISSION® pLKO.1-puro-CMV-TagRFP™ Positive Control Plasmid DNA, Contains a gene encoding TagRFP

SHC014

MISSION® pLKO.1-puro-UbC-TurboGFP™ Positive Control Plasmid DNA, Contains a gene encoding TurboGFP, under the control of the UbC promoter

Storage Class

10 - Combustible liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Visite la Librería de documentos

Zac1 regulates astroglial differentiation of neural stem cells through Socs3
Schmidt EU, et al.
Stem Cells, 31(8), 1621-1632 (2013)
Peroxiredoxin 2 nuclear levels are regulated by circadian clock synchronization in human keratinocytes
Avitabile D, et al.
The International Journal of Biochemistry & Cell Biology, 53(7580), 24-34 (2014)
Brain tumour cells interconnect to a functional and resistant network
Osswald M, et al.
Nature, 528(7580), 93-93 (2015)
Ryusuke Nakajima et al.
PloS one, 12(6), e0179585-e0179585 (2017-06-29)
A search for early response genes that are activated following germ cell induction from mouse embryonic stem cells in vitro led us to the isolation of a long noncoding RNA that contains a SINE (short interspersed element)-B1F motif that was
Sophie Weil et al.
Neuro-oncology, 19(10), 1316-1326 (2017-04-19)
Primary and adaptive resistance against chemo- and radiotherapy and local recurrence after surgery limit the benefits from these standard treatments in glioma patients. Recently we found that glioma cells can extend ultra-long membrane protrusions, "tumor microtubes" (TMs), for brain invasion
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