S9396
Sphingomyelinase from Bacillus cereus
buffered aqueous glycerol solution, ≥100 units/mg protein (Lowry)
Sinónimos:
Sphingomyelin choline phosphohydrolase, Sphingomyelin phosphodiesterase
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About This Item
Número de CAS:
MDL number:
UNSPSC Code:
12352204
NACRES:
NA.54
Productos recomendados
form
buffered aqueous glycerol solution
Quality Level
specific activity
≥100 units/mg protein (Lowry)
mol wt
34.2 kDa
foreign activity
Phospholipase C ≤1%
storage temp.
2-8°C
Gene Information
Bacillus cereus ATCC 10987 ... spH(2751530)
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General description
Sphingomyelinase (SMase) is a hydrolase, of 34.2 kDa and its active site contain as a minimum of two magnesium atoms.
Application
Sphingomyelinase from Bacillus cereus has been used:
- in the study to analyse the effect of sphingomyelinase on Rubella virus infectivity in cell lines
- in lipoprotein modification for the generation of cholesterol crystals in vitro
- to aggregate LDL (low-density lipoprotein)
Sphingomyelinase has been used in a study to assess the interaction of actin with the HIV-1 accessory protein Nef. Sphingomyelinase has also been used in a study to investigate X-ray scattering as a quality-control tool for liposomal drug-delivery systems.
Biochem/physiol Actions
Initiates the formation of sphingomyelin-based second messengers. Activates MAPK (mitogen-activated protein kinase) and SAPKs (stress-activated protein kinases); generates ceramide from sphingomyelin.
Sphingomyelin phosphodiesterase (SMPD) participates in the metabolic reactions comprising sphingomyelin. SMPD has the ability to prevent colorectal carcinoma and colitis.
Unit Definition
One unit will hydrolyze 1.0 μmole of TNPAL-sphingomyelin per min at pH 7.4 at 37 °C.
Physical form
Solution in 50% glycerol containing 50 mM Tris-HCl, pH 7.5.
Storage Class
10 - Combustible liquids
wgk_germany
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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Characterization of Sphingomyelin Phosphodiesterase Expression in Bumblebee (Bombus lantschouensis)
Han L, et al.
Journal of Insect Science, 18(5), 20-20 (2018)
Raymond Tan et al.
Experimental and molecular pathology, 94(1), 51-57 (2012-06-23)
The HIV-1 accessory protein Nef is considered to play an important role in the development of a podocyte phenotype in HIV-1 associated nephropathy. We hypothesized that Nef may be altering the podocyte phenotype both structurally and functionally. To elucidate the
Analysis of the Effect of Sphingomyelinase on Rubella Virus Infectivity in Two Cell Lines
Otsuki N, et al.
Journal of virology (2017)
Extracellular lipids accumulate in human carotid arteries as distinct three-dimensional structures and have proinflammatory properties
Lehti S, et al.
The American Journal of Pathology, 188(2), 525-538 (2018)
The Bacillus cereus group
Molecular Medical Microbiology, 1041-1078 (2015)
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