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S8633

Sigma-Aldrich

Sphingomyelinase from Staphylococcus aureus

buffered aqueous glycerol solution, 100-300 units/mg protein (Lowry)

Sinónimos:

Sphingomyelin choline phosphohydrolase, Sphingomyelin phosphodiesterase

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About This Item

Número de CAS:
9031-54-3
Comisión internacional de enzimas:
3.1.4.12 (BRENDA, IUBMB)
MDL number:
MFCD00132362
UNSPSC Code:
12352204
NACRES:
NA.32

form

buffered aqueous glycerol solution

Quality Level

200

specific activity

100-300 units/mg protein (Lowry)

storage temp.

2-8°C

Application

Sphingomyelinase from Staphylococcus aureus has been used to:
  • induce neurotoxicity in rat cortical cultures to study the protective effects of minocycline
  • determine the concentration of sphingomyelin from serum samples
  • enhance sphingomyelinase activity to study PARK9-mediated exosome biogenesis

Biochem/physiol Actions

Bacterial sphingomyelinase is active at neutral pH. When used in cell culture in vitro, it hydrolyzes the sphingomyelin on the outer leaflet of the plasma membrane and produces ceramide that is lipid-soluble. Sphingomyelinase is the key enzyme in the sphingomyelinase/ceramide pathway, which is implicated in the pathogenesis of several neurodegenerative disorders.
Initiates the formation of sphingomyelin-based second messengers. Activates MAPK (mitogen-activated protein kinase) and SAPKs (stress-activated protein kinases); generates ceramide from sphingomyelin.

Unit Definition

One unit will hydrolyze 1.0 μmol of TNPAL-sphingomyelin per min at pH 7.4 at 37 °C.

Physical form

Solution in 50% glycerol containing 0.25 M phosphate buffer, pH 7.5

Storage Class

10 - Combustible liquids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Elin Rebecka Carlsson et al.
Frontiers in endocrinology, 9, 172-172 (2018-06-21)
Metabolic surgery is superior to lifestyle intervention in reducing weight and lowering glycemia and recently suggested as treatment for type 2 diabetes mellitus. Especially Roux-en-Y gastric bypass (RYGB) has been focus for much research, but still the mechanisms of action
Measurement of sphingomyelin and ceramide cellular levels after sphingomyelinase-mediated sphingomyelin hydrolysis.
P Santana et al.
Methods in molecular biology (Clifton, N.J.), 105, 217-221 (1999-07-31)
Anne M Brauweiler et al.
The Journal of allergy and clinical immunology, 131(2), 421-427 (2012-12-19)
The skin of patients with atopic dermatitis (AD) has defects in keratinocyte differentiation, particularly in expression of the epidermal barrier protein filaggrin. AD skin lesions are often exacerbated by Staphylococcus aureus-mediated secretion of the virulence factor α-toxin. It is unknown
Lysosomal enzyme defects and Parkinson disease.
Nutan Sharma
Neurology, 80(17), 1544-1545 (2013-03-29)
Benoît-Joseph Laventie et al.
The Biochemical journal, 450(3), 559-571 (2013-01-04)
PVL (Panton-Valentine leukocidin) and other Staphylococcus aureus β-stranded pore-forming toxins are important virulence factors involved in various pathologies that are often necrotizing. The present study characterized leukotoxin inhibition by selected SCns (p-sulfonato-calix[n]arenes): SC4, SC6 and SC8. These chemicals have no
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