P4978
Phosphatase, Alkaline from calf intestine
buffered aqueous glycerol solution
Sinónimos:
CIAP, CIP, Orthophosphoric-monoester phosphohydrolase (alkaline optimum)
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About This Item
Número de CAS:
MDL number:
UNSPSC Code:
12352204
eCl@ss:
42010105
NACRES:
NA.53
Productos recomendados
grade
for molecular biology
form
buffered aqueous glycerol solution
mol wt
~80 kDa
concentration
≥10,000 units/mL
UniProt accession no.
foreign activity
DNase, RNase, none detected
shipped in
wet ice
storage temp.
−20°C
Gene Information
cow ... ALPI(280993)
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General description
Alkaline phosphatase is a phosphomonoesterase that catalyzes the cleavage of terminal phosphates from nucleic acids. The free 5′-OH can be phosphorylated with polynucleotide kinase and γ32P-ATP to produce γ32P-end-labeled nucleic acids. Linearized cloning vectors can be prevented from recircularizing by dephosphorylation with alkaline phosphatase.
Application
Commonly used to remove the 5′-terminal phosphate from nucleic acids during molecular cloning reactions.
Components
Alkaline phosphatase is provided in a solution of 10 mM Tris-HCl (pH 8.2), 50 mM KCl, 1 mM MgCl2, and 0.1 mM ZnCl2, in 50% (w/v) glycerol. 10X CIP Buffer, Product Number C3225, is included.
Unit Definition
One unit will hydrolyze 1 μmole of p-nitrophenyl phosphate per min at 37 °C.
Other Notes
Alkaline phosphatase in 5 mM EDTA (pH 8) will be irreversibly heat inactivated at 75 °C for 10 minutes.
Related product
Referencia del producto
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Precios
Glutathione S-Transferase from E. coli, recombinant, expressed in E. coli, buffered aqueous solution
Ribonucleasa A from bovine pancreas, for molecular biology, ≥70 Kunitz units/mg protein, lyophilized
inhibitor
Referencia del producto
Descripción
Precios
substrate
Referencia del producto
Descripción
Precios
Storage Class
10 - Combustible liquids
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificados de análisis (COA)
Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»
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